Klöting Nora, Klöting Ingrid
Department of Laboratory Animal Science, Medical Faculty, University of Greifswald, 17495 Karlsburg, Germany.
Biochem Biophys Res Commun. 2005 Jul 15;332(4):1070-2. doi: 10.1016/j.bbrc.2005.05.058.
Recently, Fukuhara et al. have shown that the novel visfatin is predominantly released from visceral adipocytes and shares metabolic functions with insulin. The authors suggested that there is a relationship between visfatin and the metabolic syndrome in humans. These findings prompted us to clarify the visfatin gene expression from visceral and subcutaneous adipocytes in WOKW rats, as an animal model for polygenically inherited metabolic syndrome, compared to lean Dark Agouti (DA) rats. Moreover, visfatin sequence analysis was performed in WOKW, DA rats, and disease-resistant Fisher 344, Lewis, Brown Norway, and Karlsburg wild rats. The relative gene expression of visfatin displays no significant changes in adipocytes from WOKW rats compared with DA and visfatin sequence analysis of the coding region was identical to the GenBank. But, we found length differences of two repeats, GT and GA, in intron 2 between the strains. In summary, the relative visfatin gene expression is not associated with the metabolic syndrome in WOKW rats.
最近，福原等人发现，新型内脂素主要由内脏脂肪细胞释放，并与胰岛素具有共同的代谢功能。作者认为，内脂素与人类代谢综合征之间存在关联。这些发现促使我们，以多基因遗传代谢综合征动物模型WOKW大鼠为研究对象，与瘦型黑褐大鼠（DA）对比，阐明内脏和皮下脂肪细胞中的内脂素基因表达情况。此外，还对WOKW大鼠、DA大鼠以及抗病的费希尔344大鼠、刘易斯大鼠、棕色挪威大鼠和卡尔斯堡野生大鼠进行了内脂素序列分析。与DA大鼠相比，WOKW大鼠脂肪细胞中内脂素的相对基因表达没有显著变化，编码区的内脂素序列分析结果与基因银行的数据一致。但是，我们发现不同品系大鼠的第2内含子中存在两个重复序列GT和GA的长度差异。总之，WOKW大鼠中内脂素的相对基因表达与代谢综合征无关。
