Wasylyk C, Kerckaert J P, Wasylyk B
Centre National de la Recherche Scientifique-Laboratoire de Génétique Moléculaire des Eucaryotes/Institut National de la Santé et de la Recherche Médicale (INSERM), Faculté de Médecine, Strasbourg, France.
Genes Dev. 1992 Jun;6(6):965-74. doi: 10.1101/gad.6.6.965.
The ets gene family is composed of several oncogenes and codes for transcription factors. The Ets proteins have a similar sequence called the ets domain and bind to the core motif A/CGGAA. We show here that several members of the ets family have different trans-activating properties. The ets domain of Ets-1 is required for DNA binding. Adjacent to this domain there is a novel element that inhibits DNA binding. It appears to alter the structure of the DNA-binding domain before it interacts with DNA. There is a similar sequence in Ets-2 that also inhibits DNA binding. This sequence is absent in alternative splice products of h-Ets-1. PU1, the most distantly related member of the ets gene family, lacks this novel element. It has a distinct DNA-binding specificity that is determined by DNA sequences outside the core motif. These results have important implications for both the oncogenic and normal functions of ets family members.
ets基因家族由多个癌基因组成，编码转录因子。Ets蛋白具有一个名为ets结构域的相似序列，并与核心基序A/CGGAA结合。我们在此表明，ets家族的几个成员具有不同的反式激活特性。Ets-1的ets结构域是DNA结合所必需的。在该结构域附近有一个新的元件，可抑制DNA结合。它似乎在与DNA相互作用之前改变了DNA结合结构域的结构。Ets-2中也有一个类似序列，同样抑制DNA结合。该序列在h-Ets-1的可变剪接产物中不存在。PU1是ets基因家族中亲缘关系最远的成员，缺乏这个新元件。它具有由核心基序之外的DNA序列决定的独特DNA结合特异性。这些结果对ets家族成员的致癌功能和正常功能都具有重要意义。
