Pinho G L L, da Rosa C Moura, Maciel F E, Bianchini A, Yunes J S, Proença L A O, Monserrat J M
Departamento de Ciências Fisiológicas, Fundação Universidade Federal do Rio Grande, R. Eng Alfredo Huch 475, 96201-900 Rio Grande, RS, Brazil.
Ecotoxicol Environ Saf. 2005 Jul;61(3):353-60. doi: 10.1016/j.ecoenv.2004.11.014.
Antioxidant responses and oxidative stress were evaluated in the hepatopancreas of the estuarine crab Chasmagnathus granulatus (Decapoda, Brachyura) after oral microcystin administration. Responses were evaluated through antioxidant enzyme activities (catalase-(CAT), superoxide dismutase, glutathione-S-transferase- (GST)). Nonproteic sulfhydril (NP-SH) groups, oxygen consumption, lipid peroxides (LPO), and oxidized proteins were also measured. Microcystin administration increased the oxygen consumption. GST activity and NP-SH concentration showed transient increases and CAT activity showed a peak and then a reduction. Oxidative damage was evidenced with regard to LPO content and suggested by the inhibition of CAT activity at the end of the experiment, indicating that the antioxidant response induced by the toxin was insufficient. A lowering in the number of hepatopancreatic B cells should be related to microcystin elimination.
在经口给予微囊藻毒素后，对河口蟹颗粒厚蟹（十足目，短尾亚目）肝胰腺中的抗氧化反应和氧化应激进行了评估。通过抗氧化酶活性（过氧化氢酶 -（CAT）、超氧化物歧化酶、谷胱甘肽 - S - 转移酶 -（GST））来评估反应。还测量了非蛋白巯基（NP - SH）基团、耗氧量、脂质过氧化物（LPO）和氧化蛋白。给予微囊藻毒素会增加耗氧量。GST活性和NP - SH浓度呈短暂增加，CAT活性出现一个峰值然后降低。关于LPO含量证明了氧化损伤，并且在实验结束时CAT活性的抑制表明了这一点，这表明毒素诱导的抗氧化反应不足。肝胰腺B细胞数量的减少应与微囊藻毒素的消除有关。
