TGFβ1诱导的SMAP-5基因的克隆、细胞定位、基因组结构及组织特异性表达

Cloning, cellular localization, genomic organization, and tissue-specific expression of the TGFbeta1-inducible SMAP-5 gene.

作者信息

Stolle Katrin, Schnoor Michael, Fuellen Georg, Spitzer Michael, Engel Thomas, Spener Friedrich, Cullen Paul, Lorkowski Stefan

机构信息

Institute of Arteriosclerosis Research, University of Münster, Germany.

出版信息

Gene. 2005 May 23;351:119-30. doi: 10.1016/j.gene.2005.03.012.

DOI:10.1016/j.gene.2005.03.012

PMID:15922870

Abstract

SMAP-5 is a member of the five-pass transmembrane protein family localizing in the Golgi apparatus and the endoplasmic reticulum. These proteins have been implicated in intracellular trafficking, in secretion and in vesicular transport. Phylogenetic analyses revealed that SMAP-5 is a member of a small Rab GTPase interacting factor protein family. The human SMAP-5 gene spans about 12.5 kb and comprises 6 exons on chromosomal locus 5q32. The proximal 5'-flanking region of the gene lacks a TATA box and is highly GC rich. Consistent with this, the SMAP-5 gene is expressed in all tissues. The highest level of expression was found in coronary smooth muscle cells, in which expression of the SMAP-5 gene was induced by transforming growth factor beta1, thus indicating that this protein may play an important role in inflammation.

摘要

SMAP-5是一种五跨膜蛋白家族成员，定位于高尔基体和内质网。这些蛋白质与细胞内运输、分泌和囊泡运输有关。系统发育分析表明，SMAP-5是一个小的Rab GTPase相互作用因子蛋白家族的成员。人类SMAP-5基因跨度约12.5 kb，在染色体位点5q32上包含6个外显子。该基因的近端5'侧翼区域缺乏TATA盒，富含GC。与此一致的是，SMAP-5基因在所有组织中均有表达。在冠状动脉平滑肌细胞中发现了最高水平的表达，其中SMAP-5基因的表达由转化生长因子β1诱导，因此表明该蛋白可能在炎症中起重要作用。