Pelletier Georges, Luu-The Van, Li Songyun, Labrie Fernand
Oncology and Molecular Endocrinology Research Center, Laval University Hospital (CHUL), Québec, Canada.
J Histochem Cytochem. 2005 Oct;53(10):1257-71. doi: 10.1369/jhc.5A6692.2005. Epub 2005 May 27.
The enzyme type 8 17beta-hydroxysteroid dehydrogenase (17beta-HSD) selectively catalyzes the conversion of estradiol (E2) to estrone (E1). To obtain detailed information on the sites of action of type 8 17beta-HSD, we have studied the cellular localization of type 8 17beta-HSD mRNA in mouse tissues using in situ hybridization. In the ovary, hybridization signal was detected in granulosa cells of growing follicles and luteal cells. In the uterus, type 8 17beta-HSD mRNA was found in the epithelial (luminal and glandular) and stromal cells. In the female mammary gland, the enzyme mRNA was seen in ductal epithelial cells and stromal cells. In the testis, hybridization signal was observed in the seminiferous tubule. In the prostate, type 8 17beta-HSD was detected in the epithelial cells of the acini and stromal cells. In the clitoral and preputial glands, labeling was detected in the epithelial cells of acini and small ducts. The three lobes of the pituitary gland were labeled. In the adrenal gland, hybridization signal was observed in the three zones of the cortex, the medulla being unlabeled. In the kidney, the enzyme mRNA was found to be expressed in the epithelial cells of proximal convoluted tubules. In the liver, all the hepatocytes exhibited a positive signal. In the lung, type 8 17beta-HSD mRNA was detected in bronchial epithelial cells and walls of pulmonary arteries. The present data suggest that type 8 17beta-HSD can exert its action to downregulate E2 levels in a large variety of tissues.
8型17β-羟基类固醇脱氢酶（17β-HSD）可选择性催化雌二醇（E2）转化为雌酮（E1）。为了获得有关8型17β-HSD作用位点的详细信息，我们使用原位杂交技术研究了8型17β-HSD mRNA在小鼠组织中的细胞定位。在卵巢中，在生长卵泡的颗粒细胞和黄体细胞中检测到杂交信号。在子宫中，8型17β-HSD mRNA存在于上皮细胞（腔上皮和腺上皮）和基质细胞中。在雌性乳腺中，该酶的mRNA可见于导管上皮细胞和基质细胞中。在睾丸中，在生精小管中观察到杂交信号。在前列腺中，在腺泡上皮细胞和基质细胞中检测到8型17β-HSD。在阴蒂和包皮腺中，在腺泡和小导管的上皮细胞中检测到标记。垂体的三个叶均有标记。在肾上腺中，在皮质的三个带中观察到杂交信号，髓质未标记。在肾脏中，发现该酶的mRNA在近端曲管的上皮细胞中表达。在肝脏中，所有肝细胞均显示阳性信号。在肺中，在支气管上皮细胞和肺动脉壁中检测到8型17β-HSD mRNA。目前的数据表明，8型17β-HSD可在多种组织中发挥作用以下调E2水平。
