肽缀合量子点：对活细胞中的血管紧张素1型受体进行成像。

Peptide-conjugated quantum dots: imaging the angiotensin type 1 receptor in living cells.

作者信息

Tomlinson Ian D, Mason John N, Blakely Randy D, Rosenthal Sandra J

机构信息

Department of Chemistry, Vanderbilt University, Nashville, TN, USA.

出版信息

Methods Mol Biol. 2005;303:51-60. doi: 10.1385/1-59259-901-X:051.

DOI:10.1385/1-59259-901-X:051

PMID:15923674

Abstract

Peptide-quantum dot conjugates have been prepared by attaching angiotensin II (Ang II) to cadmium selenide/zinc sulfide core-shell nanocrystals using an 1-[3-(Dimethyamino)propyl]-3-ethylcarbo diimide hydrochloride (EDC) coupling. These conjugates have been used to image angiotensin I-expressing Chinese hamster ovary (CHO) cells in vitro. When CHO cells were incubated with Ang II before incubating with Ang II-conjugated quantum dots, we were able to block the binding of the dots. The Ang II-quantum dot conjugates did not bind to parental cells and showed similar staining patterns when compared with a commercially available Ang II Alexa 488 conjugate.

摘要

通过使用1-[3-(二甲氨基)丙基]-3-乙基碳二亚胺盐酸盐（EDC）偶联剂将血管紧张素II（Ang II）连接到硒化镉/硫化锌核壳纳米晶体上，制备了肽-量子点共轭物。这些共轭物已用于体外对表达血管紧张素I的中国仓鼠卵巢（CHO）细胞进行成像。当CHO细胞在用Ang II-共轭量子点孵育之前先用Ang II孵育时，我们能够阻断量子点的结合。Ang II-量子点共轭物不与亲本细胞结合，与市售的Ang II Alexa 488共轭物相比，显示出相似的染色模式。