Guo Pei-yong, Zhu Yin-mei, Zhang Zhi-jian
Department of Environmental Science and Engineering, Huaqiao University, Quanzhou, Fujian 362021, People's Republic of China.
Luminescence. 2005 May-Jun;20(3):135-7. doi: 10.1002/bio.830.
Fine particles play an important role, not only in aquatic biogeochemical processing but also in the distribution, transfer and transformation of pollutants in the aquatic environment. Flow cytometry, widely used in biomedical research, allows fast counting and optical analysis of individual particles. Organic autotrophic particles contain naturally fluorescing pigments, such as chlorophyll and phycoerythrin. Different populations have different sizes and pigments. They also have different ratios of pigments. In general, side angle scatter (SSC) is related to the size, shape and refractive index of particles. When a 488 nm wavelength was used to excite chlorophyll and phycoerythrin fluorescence, the pigments of organic autotrophic particles emitted red and orange light. Fine particles were detected by flow cytometry (FCM) in the southern part of a eutrophic lake in winter. We found that organic autotrophic particles belonged to three populations, which represented only 15.89% of total fine particles. Organic non-living particles and inorganic particles represented the greater part (84.11%) of total fine particles. This study also demonstrated that flow cytometry is well suited to the dynamic monitoring and analysis of natural water aquatic particles that were difficult to study with traditional methods.
细颗粒不仅在水生生物地球化学过程中发挥重要作用，而且在水生环境中污染物的分布、迁移和转化方面也起着重要作用。流式细胞术在生物医学研究中广泛应用，可对单个颗粒进行快速计数和光学分析。有机自养颗粒含有天然荧光色素，如叶绿素和藻红蛋白。不同群体的颗粒大小和色素不同，色素比例也不同。一般来说，侧向散射（SSC）与颗粒的大小、形状和折射率有关。当用488nm波长激发叶绿素和藻红蛋白荧光时，有机自养颗粒的色素发出红光和橙光。冬季在一个富营养化湖泊的南部，通过流式细胞术（FCM）检测到了细颗粒。我们发现有机自养颗粒属于三个群体，仅占细颗粒总数的15.89%。有机非生物颗粒和无机颗粒占细颗粒总数的大部分（84.11%）。这项研究还表明，流式细胞术非常适合对传统方法难以研究的天然水体中的水生颗粒进行动态监测和分析。
