Chen Xi, Tao Ying, Zhao Li, Xie Zenghong, Chen Guonan
The Key Laboratory of Analytical Sciences of MOE and Department of Chemistry, Xiamen University, Xiamen 361005, People's Republic of China.
Luminescence. 2005 May-Jun;20(3):109-16. doi: 10.1002/bio.828.
Electrochemiluminescence (ECL) based on allantoin and tris(2,2'-bipyridine)ruthenium (II) [Ru(bpy)3 (2+)] was studied in aqueous alkaline buffer solution (pH 11.0). In a flowing system, the eluted allantoin was mixed with 1.0 mmol/L Ru(bpy)3 (2+). When the solution passed through a thin layer flow electrolytic cell equipped with a glassy carbon disc electrode (22.1 mm2), both hydroxyl groups of allantoin and Ru(bpy)3 (2+) were oxidized at the potential of +1.50 V (vs. Ag/AgCl). The luminescence with lambdamax 610 nm caused by the reaction of electrolytically formed Ru(bpy)3 (2+) with alkoxide radical to generate the excited state of Ru(bpy)3 (2+*). A possible ECL process of allantoin in Ru(bpy)3 (2+) alkaline solution has been discussed. In addition, the factors affecting the ECL response of allantoin are also investigated.
在pH为11.0的碱性缓冲水溶液中研究了基于尿囊素和三（2,2'-联吡啶）钌（II）[Ru(bpy)₃²⁺]的电化学发光（ECL）。在流动体系中，洗脱的尿囊素与1.0 mmol/L的Ru(bpy)₃²⁺混合。当溶液通过配备有玻碳圆盘电极（22.1 mm²）的薄层流动电解池时，尿囊素的两个羟基和Ru(bpy)₃²⁺在+1.50 V（相对于Ag/AgCl）的电位下被氧化。电解生成的Ru(bpy)₃²⁺与醇盐自由基反应产生Ru(bpy)₃²⁺*的激发态，从而导致最大发射波长为610 nm的发光。讨论了尿囊素在Ru(bpy)₃²⁺碱性溶液中可能的ECL过程。此外，还研究了影响尿囊素ECL响应的因素。
