Zhang Li, Zhang Shu-tian, Yu Zhong-lin, Shao Ying
Department of Gastroenterology, Beijing Friendship Hospital, Capital University of Medical Sciences, Beijing 100050, China.
Zhonghua Nei Ke Za Zhi. 2005 Apr;44(4):272-5.
To investigate whether the acid suppression therapy influences the absorption of bismuth from colloidal bismuth subcitrate (CBS); to locate the deposit position of bismuth in mice's organs and to detect the consequential change of cell functions in these deposited organs.
48 male SD rats weighing from 200-250 g were randomly divided into five groups: Group A(1), kill the rats on the cessation day of administration CBS; Group B(1), kill the rats on the day 8 weeks after the cessation of administration CBS; Group A(2) (CBS + amoxicillin + metronidazole + omeprazole), kill the rats on the cessation day of administration; Group B(2) (CBS + amoxicillin + metronidazole + omeprazole), kill the rats on the day 8 weeks after the cessation of administration; Control group. These medicines had been taken every day for 14 days. The issue sections (liver, brain and kidney) were counterstained after AMG development. The bismuth deposited in tissues was observed by microscopy. At the same time, the gray level of kidney tissue sections were measured and compared through image processing program. The deposition of bismuth and the degrees of cell organ's impairment were observed through electron microscopy. By the use of electron probe microanalysis, bismuth can be distinguished from chemical element.
The bismuth can be accumulated in cell bodies of proximal convoluted renal tubule, portal area, hypothalamus, and hypoglossal nuclei after its absorption. Under the light microscopy, heavy AMG staining granules were found in cell bodies of proximal convoluted renal tubule. It was discovered that the amounts of bismuth accumulation in kidney of quadruple therapy group were much more than that of single compound therapy group (P < 0.05). The amounts of bismuth accumulation in kidney on the cessation day of administration are more than that 8 weeks later (P < 0.01). What is more, under the electron microscopy, heavy AMG staining granules were found exclusively in lysosomes of proximal convoluted renal tubule cell. The electron microscopy found some cell impairment in quadruple therapy group: the impairment to these cells can be recovered 8 weeks after the cessation of administration.
The acid suppression therapy causes an increase of bismuth absorption and accumulation from CBS in the rats' kidney. Finally, the absorbed bismuth can be discharged out of the body via kidney. Large amounts of bismuth accumulation in kidney can impair the functions of proximal convoluted renal tubule cell.
研究抑酸治疗是否会影响枸橼酸铋钾（CBS）中铋的吸收；确定铋在小鼠器官中的沉积位置，并检测这些沉积器官中细胞功能的相应变化。
将48只体重200 - 250 g的雄性SD大鼠随机分为五组：A（1）组，在停止给予CBS当天处死大鼠；B（1）组，在停止给予CBS后8周处死大鼠；A（2）组（CBS + 阿莫西林 + 甲硝唑 + 奥美拉唑），在停止给药当天处死大鼠；B（2）组（CBS + 阿莫西林 + 甲硝唑 + 奥美拉唑），在停止给药后8周处死大鼠；对照组。这些药物每天服用14天。经AMG显色后对组织切片（肝脏、脑和肾脏）进行复染。通过显微镜观察组织中沉积的铋。同时，通过图像处理程序测量并比较肾组织切片的灰度值。通过电子显微镜观察铋的沉积情况以及细胞器的损伤程度。利用电子探针微分析可区分铋与化学元素。
铋吸收后可在近端肾小管细胞体、门管区、下丘脑和舌下神经核中蓄积。在光学显微镜下，近端肾小管细胞体中发现大量AMG染色颗粒。发现四联疗法组肾脏中铋的蓄积量远多于单一复方疗法组（P < 0.05）。给药停止当天肾脏中铋的蓄积量多于8周后（P < 0.01）。此外，在电子显微镜下，仅在近端肾小管细胞的溶酶体中发现大量AMG染色颗粒。电子显微镜观察到四联疗法组存在一些细胞损伤：给药停止8周后这些细胞的损伤可恢复。
抑酸治疗导致大鼠肾脏中CBS的铋吸收和蓄积增加。最终，吸收的铋可通过肾脏排出体外。肾脏中大量铋的蓄积会损害近端肾小管细胞的功能。
