Li Guang-Yu, Fan Bin, Wu Ya-Zhen, Wang Xin-Rui, Wang Yao-Hui, Wu Jia-Xiang
Department of Ophthalmology, the Second Hospital of Jilin University, Changchun 130041, China.
Zhonghua Yan Ke Za Zhi. 2005 Apr;41(4):312-6.
To investigate the expression of HIF-1alpha in human retinal pigment epithelium (hRPE) induced by hypoxia at different time points and to study the mechanism of choroidal neovascularization.
hRPE were isolated, cultured and identified. The changes of level of mRNA and protein of HIF-1alpha in hRPE at different time points after exposed to hypoxia were measured by semi-quantitative RT-PCR and immunofluorescence techniques.
A significant increase of HIF-1alpha level in cultured hRPE was induced by hypoxia. After 8 hours exposed to hypoxia, the level of HIF-1alpha in the hRPE reached a peak and sustained for more than 16 hours. After a prolonged exposure to hypoxia, the morphology of hRPE began to change and the HIF-1alpha level was decreased.
The level of HIF-1alpha in hRPE rises obviously after exposed to hypoxia. This may play a role in the occurrence of choroidal neovascularization.
研究缺氧不同时间点对人视网膜色素上皮细胞（hRPE）中缺氧诱导因子-1α（HIF-1α）表达的影响，探讨脉络膜新生血管形成的机制。
分离、培养及鉴定hRPE。采用半定量逆转录-聚合酶链反应（RT-PCR）和免疫荧光技术检测缺氧不同时间点hRPE中HIF-1α mRNA及蛋白水平的变化。
缺氧可诱导培养的hRPE中HIF-1α水平显著升高。缺氧8小时后，hRPE中HIF-1α水平达到峰值，并持续16小时以上。长时间缺氧后，hRPE形态开始改变，HIF-1α水平下降。
hRPE暴露于缺氧环境后HIF-1α水平明显升高，这可能在脉络膜新生血管形成中起作用。
