Chen Xue-Guo, He Shou-Zhi
Department of Ophthalmology, PLA Genenal Hospital, Beijing 100853, China.
Zhonghua Yan Ke Za Zhi. 2005 Apr;41(4):317-20.
To explore the gene expression patterns of choroidal neovascularization with gene chip technology.
Total RNA was isolated from normal choroid and choroid tissues with neovascularization induced by krypton laser photocoagulation in Brown Norway rat. The mRNA was purified and was reversely transcribed to cDNA to prepare for hybridization probes. In choroidal neovascularization, the differentially expressed genes were assayed with gene chips which contained 5705 rat genes.
There were 46 genes expressed differentially in choroidal neovascularization. Of these, the expression of 3 genes was upregulated and 43 genes downregulated. These genes were involved in the function of transport, signal, metabolism, development and differentiation of cell adhesion.
Numerous genes are involved in the development of choroidal neovascularization induced by krypton laser photocoagulation.
应用基因芯片技术探讨脉络膜新生血管的基因表达谱。
从正常脉络膜及经氪激光光凝诱导产生新生血管的棕色挪威大鼠脉络膜组织中提取总RNA。纯化mRNA并逆转录成cDNA以制备杂交探针。在脉络膜新生血管中，用包含5705个大鼠基因的基因芯片检测差异表达基因。
脉络膜新生血管中有46个基因差异表达。其中，3个基因表达上调，43个基因表达下调。这些基因涉及转运、信号传导、代谢、细胞黏附的发育和分化功能。
许多基因参与氪激光光凝诱导的脉络膜新生血管的发生。
