Dai Yi, Sun Xing-Huai
Department of Ophthalmology, Eye & ENT Hospital, Fudan University, Shanghai 200031, China.
Zhonghua Yan Ke Za Zhi. 2005 Apr;41(4):321-4.
To investigate the early neuronal response to optic nerve crush in the lateral geniculate nucleus (LGN) of rats.
Unilateral optic nerve crush injury was produced in Sprague-Dawley rats by using micro clips of 70 gram forces. The rats were sacrificed at time periods of 2 hours, 1, 3, 7 and 14 days after crush. C-fos expression was studied with a polyclonal antibody by immunohistochemistry and Western blot. Double immunolabelling of c-fos and calbindin D-28k was also studied by confocal laser scanning microscope.
Expression of c-fos was observed ipslaterally in the medial subdivision of ventral LGN and intermediate geniculate nucleus (IGN). The numbers of c-fos positive cells were visible at 2 h post injury and peaked at 1 d. Thereafter, the expression decreased at 3 d, 7 d and still a few was observed at 14 d. C-fos immunoreactivity was scarcely observed in the dorsal LGN and in the LGN of control rats. Expression of calbindin D-28k was mainly observed in the medial subdivision of ventral LGN and IGN. 75% approximately 83% c-fos positive cells were also calbindin D-28k immunopositive.
Expression of c-fos was induced in calbindin D-28k positive relay neurons in the ipslaterally medial subdivision of ventral LGN and IGN. They may play some important roles during the pathologic process of acute optic nerve injury.
研究大鼠外侧膝状体（LGN）对视神经挤压的早期神经元反应。
使用70克力的微型夹在Sprague-Dawley大鼠中造成单侧视神经挤压损伤。在挤压后2小时、1天、3天、7天和14天的时间段处死大鼠。通过免疫组织化学和蛋白质印迹法用多克隆抗体研究C-fos表达。还通过共聚焦激光扫描显微镜研究C-fos和钙结合蛋白D-28k的双重免疫标记。
在腹侧LGN内侧亚区和中间膝状体核（IGN）同侧观察到C-fos表达。损伤后2小时可见C-fos阳性细胞数量，1天时达到峰值。此后，在3天、7天时表达下降，14天时仍观察到少量表达。在背侧LGN和对照大鼠的LGN中几乎未观察到C-fos免疫反应性。钙结合蛋白D-28k表达主要在腹侧LGN内侧亚区和IGN中观察到。约75%至83%的C-fos阳性细胞也是钙结合蛋白D-28k免疫阳性。
在腹侧LGN内侧亚区和IGN同侧的钙结合蛋白D-28k阳性中继神经元中诱导了C-fos表达。它们可能在急性视神经损伤的病理过程中发挥一些重要作用。
