Shi Wei-Yun, Xie Li-Xin
Qingdao Eye Hospital,Shandong Eye Institute, Qingdao 266071, China.
Zhonghua Yan Ke Za Zhi. 2005 Apr;41(4):350-4.
To study the mechanism and the features of corneal allograft rejection using a model of CD4 and CD8 knock-out mice.
The mice were divided into three groups with 20 mice in each group. CD4 knock-out mice, CD8 knock-out mice and C57BL/6 mice were used as recipients and BALB/c mice as corneal graft donors. Postoperative evaluation included slit-lamp biomicroscopy and immunohistological studies. The rejection times were recorded and the cytokines in the eye after the surgery were measured at 1 week, 2 weeks and 4 weeks after the transplantation. Thirty mice (each group ten mice) received skin transplantation using BALB/c as donors in the second week after penetrating keratoplasty. The time of skin grafts rejection was recorded and the cornea grafts were inspected when the skin grafts were rejected.
The rejection time varied in these three groups. The corneal grafts in CD4 knock-out mice were clear and no rejection occurred > 90 days. The corneal grafts in CD8 knock out mice were rejected at (28 +/- 3) days. The grafts in the control groups were rejected at (14 +/- 2) days (F = 2034, P < 0.01). The skin grafts rejection were recorded at (14 +/- 2), (12 +/- 1), (10 +/- 1) days in CD4 knock-out mice, CD8 knock-out mice and control groups, respectively (F = 42.54, P < 0.01).
CD4 and CD8 knock-out mice are useful models for studying of corneal graft rejection. The cornea allograft rejection after penetrating keratoplasty is mediated primarily by CD(4)(+) T lymphocytes. The CD(8)(+) T lymphocytes also participate in the rejection processes.
利用CD4和CD8基因敲除小鼠模型研究角膜移植排斥反应的机制和特点。
将小鼠分为三组，每组20只。以CD4基因敲除小鼠、CD8基因敲除小鼠和C57BL/6小鼠作为受体，BALB/c小鼠作为角膜移植供体。术后评估包括裂隙灯生物显微镜检查和免疫组织学研究。记录排斥反应时间，并在移植后1周、2周和4周测量术后眼部的细胞因子。30只小鼠（每组10只）在穿透性角膜移植术后第2周接受以BALB/c小鼠为供体的皮肤移植。记录皮肤移植排斥反应时间，并在皮肤移植排斥时检查角膜移植片。
这三组的排斥反应时间各不相同。CD4基因敲除小鼠的角膜移植片清晰，90天以上未发生排斥反应。CD8基因敲除小鼠的角膜移植片在（28±3）天被排斥。对照组的移植片在（14±2）天被排斥（F = 2034，P < 0.01）。CD4基因敲除小鼠、CD8基因敲除小鼠和对照组的皮肤移植排斥反应时间分别记录为（14±2）、（12±1）、（10±1）天（F = 42.54，P < 0.01）。
CD4和CD8基因敲除小鼠是研究角膜移植排斥反应的有用模型。穿透性角膜移植术后的角膜移植排斥反应主要由CD4(+) T淋巴细胞介导。CD8(+) T淋巴细胞也参与排斥反应过程。
