Mileykovskiy Boris Y, Kiyashchenko Lyudmila I, Siegel Jerome M
Department of Psychiatry and Biobehavioral Sciences, University of California, Los Angeles, Los Angeles, California 90095, USA.
Neuron. 2005 Jun 2;46(5):787-98. doi: 10.1016/j.neuron.2005.04.035.
Micropipette recording with juxtacellular Neurobiotin ejection, linked micropipette-microwire recording, and antidromic and orthodromic activation from the ventral tegmental area and locus coeruleus were used to identify hypocretin (Hcrt) cells in anesthetized rats and develop criteria for identification of these cells in unanesthetized, unrestrained animals. We found that Hcrt cells have broad action potentials with elongated later positive deflections that distinguish them from adjacent antidromically identified cells. They are relatively inactive in quiet waking but are transiently activated during sensory stimulation. Hcrt cells are silent in slow wave sleep and tonic periods of REM sleep, with occasional burst discharge in phasic REM. Hcrt cells discharge in active waking and have moderate and approximately equal levels of activity during grooming and eating and maximal activity during exploratory behavior. Our findings suggest that these cells are activated during emotional and sensorimotor conditions similar to those that trigger cataplexy in narcoleptic animals.
使用带有细胞旁神经生物素喷射的微吸管记录、连接微吸管-微丝记录以及来自腹侧被盖区和蓝斑的逆向和顺向激活，来识别麻醉大鼠中的下丘脑泌素（Hcrt）细胞，并制定在未麻醉、不受束缚动物中识别这些细胞的标准。我们发现，Hcrt细胞具有宽动作电位，后期有延长的正向偏转，这使其与相邻的逆向识别细胞区分开来。它们在安静觉醒时相对不活跃，但在感觉刺激期间会短暂激活。Hcrt细胞在慢波睡眠和快速眼动睡眠的紧张期是沉默的，在快速眼动睡眠的相位期偶尔会有爆发性放电。Hcrt细胞在活跃觉醒时放电，在梳理毛发和进食期间具有中等且大致相等的活动水平，在探索行为期间具有最大活动水平。我们的研究结果表明，这些细胞在类似于引发发作性睡病动物猝倒的情绪和感觉运动状态下被激活。
