Malina Robin, Motoyama Satoru, Hamana Shinya, Maruo Takeshi
Department of Obstetrics and Gynecology, Kobe University Graduate School of Medicine, Kobe, Japan.
Kobe J Med Sci. 2004;50(3-4):123-30.
Laminin-5 is an essential component of epithelial basal membranes. Matrix metalloproteinase-2 (MMP-2) changes laminin-5 from static to migratory substrate by cleaving the short arm of the gamma2 subunit of the laminin-5. Recently, the expression of laminin-5 gamma2 chain has been reported to be a marker of invasiveness of epithelial malignant tumors. We investigated the expression of laminin-5 gamma2 chain and MMP-2 in the neoplastic changes of uterine cervical squamous epithelium. Tissue samples of normal uterine cervix, cervical intraepithelial neoplasia-1, -3 (CIN-1, -3) and squamous cell carcinoma keratinizing (SCC-K) and non-keratinizing (SCC-NK) were analyzed for expression of the laminin-5 gamma2 chain and MMP-2 by immunohistochemistry. In normal uterine cervix and CIN-1, laminin-5 gamma2 chain was immunolocalized only in the basal membrane. In CIN-3, expression of laminin-5 gamma2 chain and MMP-2 became apparent at the epithelial-stromal interface of epithelial cells. In SCC-K and SCC-NK, laminin-5 gamma2 chain and MMP-2 expression were abundant at the epithelial-stromal interface of tumor clusters. These results suggest that the expression of laminin-5 gamma2 chain and MMP-2 may be involved in the process of neoplastic changes of uterine cervical squamous epithelium.
层粘连蛋白-5是上皮基底膜的重要组成部分。基质金属蛋白酶-2（MMP-2）通过切割层粘连蛋白-5γ2亚基的短臂，将层粘连蛋白-5从静态底物转变为迁移底物。最近，有报道称层粘连蛋白-5γ2链的表达是上皮恶性肿瘤侵袭性的标志物。我们研究了层粘连蛋白-5γ2链和MMP-2在子宫颈鳞状上皮肿瘤性变化中的表达。通过免疫组织化学分析正常子宫颈、宫颈上皮内瘤变1级、3级（CIN-1、-3）以及角化型（SCC-K）和非角化型（SCC-NK）鳞状细胞癌组织样本中层粘连蛋白-5γ2链和MMP-2的表达。在正常子宫颈和CIN-1中，层粘连蛋白-5γ2链仅免疫定位在基底膜。在CIN-3中，层粘连蛋白-5γ2链和MMP-2的表达在上皮细胞的上皮-间质界面处变得明显。在SCC-K和SCC-NK中，层粘连蛋白-5γ2链和MMP-2的表达在肿瘤细胞团的上皮-间质界面处丰富。这些结果表明，层粘连蛋白-5γ2链和MMP-2的表达可能参与子宫颈鳞状上皮肿瘤性变化的过程。
