Li Zhi-li, Yuan Ming, Jiang Shi-zhong, Wang De-sheng, Wang Hui-juan
Space Med Med Eng (Beijing). 2004 Oct;17(5):330-3.
To determine the role of Rho associated kinase (ROK) in contraction of rat femoral arteries after 14 d tail suspension.
Male Wistar rats were randomly divided into control group (CON) and 14 d -30 degrees tail suspension group (TS14d). Analysis was performed on the contractile responses of perfused femoral arterial rings from both TS14d and CON rats to KCl, phenylephrine (PE), and U-46619 in the presence of Y-27632.
Arterial rings from TS14d rats displayed a reduced contractile response to KCl and PE but not significantly to U-46619. In the response to KCl, Y-27632 caused a concentration-dependent relaxation and significantly larger decrease of contraction in tissues from TS14d rats. Y27632 nearly abolished the tonic component of KCl-induced contraction when its dose was increased from 10(-6)mol/L to 10(-5) mol/L. It had also an inhibitive effect on the PE and U-46619-induced contraction and caused significantly larger decrease in U-46619 but not in KCl or PE induced contraction in tissues from TS14d rats.
ROK activity may be enhanced and play a compensational role in rat femoral arteries after TS14d.
确定Rho相关激酶（ROK）在大鼠尾部悬吊14天后股动脉收缩中的作用。
将雄性Wistar大鼠随机分为对照组（CON）和尾部悬吊14天 -30度组（TS14d）。在存在Y-27632的情况下，对TS14d组和CON组大鼠的灌注股动脉环对氯化钾、去氧肾上腺素（PE）和U-46619的收缩反应进行分析。
TS14d组大鼠的动脉环对氯化钾和PE的收缩反应降低，但对U-46619的反应无明显降低。在对氯化钾的反应中，Y-27632引起浓度依赖性舒张，并且在TS14d组大鼠的组织中收缩的降低明显更大。当Y27632的剂量从10（-6）mol/L增加到10（-5）mol/L时，它几乎消除了氯化钾诱导收缩的张力成分。它对PE和U-46619诱导的收缩也有抑制作用，并且在TS14d组大鼠的组织中对U-46619诱导的收缩降低明显更大，但对氯化钾或PE诱导的收缩无明显降低。
TS14d后大鼠股动脉中ROK活性可能增强并发挥代偿作用。
