Serov O L, Matveeva N M, Kuznetsov S B, Kaftanovskaia E M
Izv Akad Nauk Ser Biol. 2001 Nov-Dec(6):711-6.
Here we study the properties of cell hybrids produced by the fusion of embryonal stem cells and differentiated ones. During in vitro cultivation, such hybrids predominantly lose the somatic partner chromosomes, although the loss of the embryonic partner autosomes 1, 9, 11, 12, 15, 16, 18, and 19 is also common in the clones; i.e., this is a bidirectional process. The use of a selective media allows the isolation of the clones, with the embryonal X chromosome replaced by the somatic genome homolog. The cell hybrids with a near-diploid chromosome set preserve the high-level pluripotency properties of the embryonal partner including the capacity to form chimeras after their introduction in the blastocoel. An investigation of the chimeric animals demonstrated a reprogramming of the "somatic" X chromosome in the course of development. The prospective identification of the chromosomes involved in the maintenance of pluripotency and studies of its cis- and trans-regulation in the cell hybrid genome are discussed.
在此，我们研究胚胎干细胞与分化细胞融合产生的细胞杂种的特性。在体外培养过程中，此类杂种主要会丢失体细胞伴侣染色体，不过在克隆中胚胎伴侣常染色体1、9、11、12、15、16、18和19的丢失也很常见；也就是说，这是一个双向过程。使用选择性培养基可分离出克隆，其中胚胎X染色体被体细胞基因组同源物取代。具有近二倍体染色体组的细胞杂种保留了胚胎伴侣的高水平多能性特性，包括在被引入囊胚腔后形成嵌合体的能力。对嵌合动物的研究表明，“体细胞”X染色体在发育过程中发生了重编程。文中讨论了对维持多能性所涉及染色体的前瞻性鉴定及其在细胞杂种基因组中的顺式和反式调控研究。
