Cateau Estelle, Tournier Nicolas, Dupuis Antoine, Le Moal Gwenaël, Venisse Nicolas
Service de Pharmacocinétique, CHU Poitiers, BP 577, 86021 Poitiers Cedex, France.
J Pharm Biomed Anal. 2005 Sep 15;39(3-4):791-5. doi: 10.1016/j.jpba.2005.04.025.
Atazanavir is a new HIV-1 protease inhibitor. A simple high-performance liquid chromatographic method using UV detection was developed and validated for the analysis of atazanavir in human plasma. The sample clean up was carried out using solid-phase extraction with OASIS MCX cartridge. The chromatographic separation was achieved on a Kromasil C18 (150 mm x 3 mm, 5 microm) column with a mobile phase consisting of acetonitrile and water (38:62 v/v) delivered isocratically. The effluent of the column was monitored at a wavelength of 210 nm. The assay was linear over the concentration range of 0.156 to 10 microg/ml and the limit of quantification was 0.156 microg/ml. The method was also validated with respect to recovery, precision, accuracy and specificity. This method is suitable for therapeutic drug monitoring of atazanavir and can be easily reproduced with standard equipment.
阿扎那韦是一种新型HIV-1蛋白酶抑制剂。建立了一种使用紫外检测的简单高效液相色谱法，并对其在人血浆中分析阿扎那韦的方法进行了验证。使用OASIS MCX柱进行固相萃取以净化样品。在Kromasil C18（150 mm×3 mm，5微米）柱上进行色谱分离，流动相由乙腈和水（38:62 v/v）等度洗脱组成。在210 nm波长下监测柱流出物。该测定法在0.156至10微克/毫升的浓度范围内呈线性，定量限为0.156微克/毫升。还对该方法的回收率、精密度、准确度和特异性进行了验证。该方法适用于阿扎那韦的治疗药物监测，并且使用标准设备即可轻松重现。
