护士鲨（Ginglymostoma cirratum）和星鼻魟（Raja eglanteria）外周血白细胞产生一氧化氮的情况。
Nitric oxide production by nurse shark (Ginglymostoma cirratum) and clearnose skate (Raja eglanteria) peripheral blood leucocytes.
作者信息
Walsh Cathy J, Toranto Jason D, Gilliland C Taylor, Noyes David R, Bodine Ashby B, Luer Carl A
机构信息
Mote Marine Laboratory, Sarasota, FL 34236, USA.
出版信息
Fish Shellfish Immunol. 2006 Jan;20(1):40-6. doi: 10.1016/j.fsi.2005.03.011.
Reactive nitrogen intermediates, such as nitric oxide (NO), are important immunomodulators in vertebrate immune systems, but have yet to be identified as mediators of host defence in any member of class Chondrichthyes, the cartilaginous fishes. In the present study, production of NO by nurse shark (Ginglymostoma cirratum) peripheral blood leucocytes (PBL) stimulated with bacterial cell wall lipopolysaccharide (LPS) was investigated. PBL were cultured for 24 to 96 h following stimulation with LPS at concentrations ranging from 0 to 25 microg ml(-1), in both serum-supplemented and serum-free culture conditions. Production of NO was measured indirectly using the Griess reaction, with maximal NO production occurring after 72 h using 10% FBS and 10 microg LPS ml(-1). Application of these culture conditions to PBL from another cartilaginous fish (clearnose skate, Raja eglanteria) resulted in a similar NO response. Addition of a specific inhibitor of inducible nitric oxide synthase (iNOS), L-N(6)-(1-iminoethyl)lysine (L-NIL), resulted in a significant decrease in the production of NO by PBL from both species.
反应性氮中间产物，如一氧化氮（NO），是脊椎动物免疫系统中的重要免疫调节剂，但在软骨鱼类（Chondrichthyes）的任何成员中，尚未被确定为宿主防御的介质。在本研究中，研究了用细菌细胞壁脂多糖（LPS）刺激护士鲨（Ginglymostoma cirratum）外周血白细胞（PBL）产生NO的情况。在添加血清和无血清培养条件下，用浓度范围为0至25μg/ml（-1）的LPS刺激PBL，并培养24至96小时。使用Griess反应间接测量NO的产生，在使用10%胎牛血清和10μg LPS/ml（-1）的情况下，72小时后NO产生量最大。将这些培养条件应用于另一种软骨鱼类（清洁鳐，Raja eglanteria）的PBL，产生了类似的NO反应。添加诱导型一氧化氮合酶（iNOS）的特异性抑制剂L-N（6）-（1-亚氨基乙基）赖氨酸（L-NIL），导致这两个物种的PBL产生的NO显著减少。
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