Itoh N, Hanafusa T, Miyagawa J, Tamura S, Inada M, Kawata S, Kono N, Tarui S
Second Department of Internal Medicine, Osaka University Medical School, Japan.
J Clin Endocrinol Metab. 1992 Jun;74(6):1372-7. doi: 10.1210/jcem.74.6.1592883.
We investigated transthyretin (TTR) in the pancreases and sera of 10 newly diagnosed type I diabetic patients by immunohistochemistry and nephelometry. In the type I diabetic pancreases, glucagon-positive A-cells showed strong immunoreactivity for TTR, the intensity and distribution pattern of which corresponded to those in normal subjects. Morphometric analysis revealed that the amount of strongly TTR-positive A-cells was not significantly different from that in normal subjects. On the contrary, insulin-positive B-cells, which normally show uneven and weak TTR immunoreactivity, decreased in number, and only a few residual B-cells showed faint immunoreactivity. Neither somatostatin cells nor pancreatic polypeptide cells were positive for TTR. The serum TTR concentration showed a significant decrease in type I diabetic patients compared with that in normal subjects (P less than 0.005). These data suggest that the synthesis or storage of TTR in A-cells is not affected, but that in B-cells is impaired in type I diabetes. The decrease in serum TTR might be one of the features of metabolic disorders in type I diabetes.
我们通过免疫组织化学和散射比浊法研究了10例新诊断的I型糖尿病患者胰腺和血清中的转甲状腺素蛋白（TTR）。在I型糖尿病患者的胰腺中，胰高血糖素阳性的A细胞对TTR显示出强免疫反应性，其强度和分布模式与正常受试者的相符。形态计量分析显示，TTR强阳性A细胞的数量与正常受试者相比无显著差异。相反，正常情况下显示TTR免疫反应性不均匀且较弱的胰岛素阳性B细胞数量减少，仅少数残留的B细胞显示微弱的免疫反应性。生长抑素细胞和胰多肽细胞均未显示TTR阳性。与正常受试者相比，I型糖尿病患者的血清TTR浓度显著降低（P<0.005）。这些数据表明，I型糖尿病中A细胞中TTR的合成或储存未受影响，但B细胞中的TTR合成或储存受损。血清TTR降低可能是I型糖尿病代谢紊乱的特征之一。
