基质金属蛋白酶-2 mRNA表达与人胃癌临床病理、尿激酶型纤溶酶原激活物系统参数及预后的关系
Relationship between matrix metalloproteinase-2 mRNA expression and clinicopathological and urokinase-type plasminogen activator system parameters and prognosis in human gastric cancer.
作者信息
Ji Feng, Chen Yue-Liang, Jin En-Yun, Wang Wei-Lin, Yang Zi-Li, Li You-Ming
机构信息
Department of Gastroenterology, First Affiliated Hospital of Medical College, Zhejiang University, Qingchun Road 79, Hangzhou 310003, Zhejiang Province, China.
出版信息
World J Gastroenterol. 2005 Jun 7;11(21):3222-6. doi: 10.3748/wjg.v11.i21.3222.
AIM
To investigate the relationship between matrix metalloproteinase-2 (MMP-2) mRNA expression and clinicopathologic and urokinase-type plasminogen activator (uPA) system parameter and prognosis in human gastric cancer.
METHODS
Expression of MMP-2 mRNA, uPA, and uPA-R mRNA in tumor tissues and > or =5 cm adjacent normal tissues from 67 cases of gastric cancer was studied using RT-PCR and Northern blot respectively. Survival analyses were done using the Kaplan-Meier method.
RESULTS
The expression rates of MMP-2 mRNA, uPA and uPA-R mRNA in tumor tissues (31%, 41%, and 51%, respectively) were significantly higher than those in > or =5 cm adjacent tissues (19%, 11%, and 9%; chi(2) = 4.59, 43.58, and 53.24 respectively, P<0.05, 0.0001, and 0.0001, respectively). Expression of MMP-2 mRNA was significantly correlated with lymph node metastasis (metastasis: 61.9%, no metastasis: 39.1%, chi(2) = 7.61, P<0.05), Lauren's classification of diffuse/mixed types: 54.2%, intestinal type: 26.3%, chi(2) = 4.25, P<0.05, expression of uPA and uPA-R mRNA (uPA+: 55.1%, uPA-: 22.2% and uPA-R+: 54.9%, uPA-R-: 18.8%, chi(2) = 5.72 and 6.40 respectively, P<0.05). Kaplan-Meier survival analysis of MMP-2 mRNA expression did not show significant difference in all 67 cases, but revealed an association of the expression of MMP-2 mRNA, uPA, and uPA-R mRNA with worse prognosis (P = 0.0083, 0.0160, and 0.0094, respectively).
CONCLUSION
MMP-2 may play an important role in the development of invasion and metastasis of gastric cancer.
目的
探讨基质金属蛋白酶-2（MMP-2）mRNA表达与人类胃癌临床病理、尿激酶型纤溶酶原激活物（uPA）系统参数及预后之间的关系。
方法
分别采用逆转录-聚合酶链反应（RT-PCR）和Northern印迹法研究67例胃癌患者肿瘤组织及距肿瘤边缘≥5 cm的癌旁正常组织中MMP-2 mRNA、uPA及uPA-R mRNA的表达情况。采用Kaplan-Meier法进行生存分析。
结果
肿瘤组织中MMP-2 mRNA、uPA及uPA-R mRNA的表达率分别为31%、41%和51%，显著高于距肿瘤边缘≥5 cm的癌旁组织（分别为19%、11%和9%；χ²分别为4.59、43.58和53.24，P值分别<0.05、0.0001和0.0001）。MMP-2 mRNA的表达与淋巴结转移显著相关（转移：61.9%，无转移：39.1%，χ² = 7.61，P<0.05），与Lauren分类的弥漫/混合型相关（54.2%），与肠型相关（26.3%），χ² = 4.25，P<0.05，与uPA及uPA-R mRNA的表达相关（uPA阳性：55.1%，uPA阴性：22.2%；uPA-R阳性：54.9%，uPA-R阴性：18.8%，χ²分别为5.72和6.40，P<0.05）。对所有67例患者进行的MMP-2 mRNA表达的Kaplan-Meier生存分析未显示出显著差异，但显示MMP-2 mRNA、uPA及uPA-R mRNA的表达与较差的预后相关（P值分别为0.0083、0.0160和0.0094）。
结论
MMP-2可能在胃癌侵袭和转移的发生发展中起重要作用。
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