Tang Kung-Sheng, Chiu Hui-Fen, Chen Hong-Hwa, Eng Hock-Liew, Tsai Chia-Jung, Teng Hsiu-Chen, Huang Ching-Shan
Department of Medical Technology, Fooyin University, 151 Chin-Hsueh Road, Ta-Liao Hsiang, Kaohsiung Hsien 831, Taiwan, China.
World J Gastroenterol. 2005 Jun 7;11(21):3250-4. doi: 10.3748/wjg.v11.i21.3250.
To investigate the relationship between single nucleotide polymorphisms in the uridine-diphosphoglucuronosyltransferase (UGT) UGT1A7 and UGT1A1 genes and patients suffering from colorectal cancer (CRC).
A case-control study was designed in order to investigate the genotypes of the UGT1A7 and UGT1A1 genes, which were identified by the polymerase chain reaction-restriction fragment length polymorphism (RFLP) method, for 268 CRC patients and 441 healthy controls.
The results of simple logistical regressions revealed odds ratios (ORs) of 1.97 (P<0.001), 1.91 (P<0.001), and 2.03 (P<0.001) for patients who carried the UGT1A71/3 genotype, UGT1A73 allele, and variant-211 UGT1A1 allele. The interaction of UGT1A73 allele and variant-211 UGT1A1 allele produced an additive effect on the risk for the development of CRC (observed OR (2.34) greater than expected OR (1.59)). For the 268 patients, the results of simple logistical regressions indicated that the OR of developing metastases was 4.90 (P<0.001) and 4.89 (P<0.001) for the individuals possessing UGT1A7*3 allele and variant-211 UGT1A1 allele, respectively. The results of multivariate logistical regressions confirmed these findings (OR = 2.51, P = 0.01; and OR = 2.71, P = 0.01, respectively). The interaction of these two variants resulted in an additive effect on the risk for metastases amongst patients (observed OR (6.83) greater than expected OR (4.56)).
In conclusion, carriage of the UGT1A7*3 allele, as well as variant-211 UGT1A1 allele represents a risk factor for the development of, and a determinant for, metastases associated with CRC patients.
研究尿苷二磷酸葡萄糖醛酸转移酶（UGT）UGT1A7和UGT1A1基因中的单核苷酸多态性与结直肠癌（CRC）患者之间的关系。
设计了一项病例对照研究，采用聚合酶链反应-限制性片段长度多态性（RFLP）方法，对268例CRC患者和441例健康对照者的UGT1A7和UGT1A1基因进行基因分型。
简单逻辑回归结果显示，携带UGT1A71/3基因型、UGT1A73等位基因和UGT1A1基因-211变体等位基因的患者的优势比（OR）分别为1.97（P<0.001）、1.91（P<0.001）和2.03（P<0.001）。UGT1A73等位基因与UGT1A1基因-211变体等位基因的相互作用对CRC发生风险产生累加效应（观察到的OR（2.34）大于预期OR（1.59））。对于这268例患者，简单逻辑回归结果表明，携带UGT1A7*3等位基因和UGT1A1基因-211变体等位基因的个体发生转移的OR分别为4.90（P<0.001）和4.89（P<0.001）。多变量逻辑回归结果证实了这些发现（OR分别为2.51，P = 0.01；以及OR为2.71，P = 0.01）。这两种变体的相互作用对患者转移风险产生累加效应（观察到的OR（6.83）大于预期OR（4.56））。
总之，携带UGT1A7*3等位基因以及UGT1A1基因-211变体等位基因是CRC患者发生转移的一个风险因素和决定因素。
