Arends J, ten Bosch J J
Laboratory for Materia Technica, University of Groningen, The Netherlands.
J Dent Res. 1992 Apr;71 Spec No:924-8. doi: 10.1177/002203459207100S27.
This paper compares the experimental techniques utilized to assess the de- or remineralization of enamel or dentin in intra-oral studies. In in situ studies, it is important for one to know how much mineral has been lost or gained, and where the loss or gain occurred. The main emphasis in this paper is on techniques suitable for direct or indirect mineral quantification. The measuring techniques considered are microradiography, iodine absorptiometry, various microhardness tests, polarized light, light-scattering, iodide permeability, and wet chemical analysis. The various techniques are compared concerning suitability for the determination of mineral content in vol% (or wt%), mineral changes in vol% micron (or kg.m-2), and mineral distributions. Furthermore, sample preparation, the importance of protein penetration, nominal mineral loss range, the estimated mineral loss threshold, and the applicability to dentin are compared and considered. It is concluded that, although more than ten techniques are available for the measurement of changes after de- or remineralization in situ, transverse microradiography is the most practical technique for the direct and quantitative measurement of mineral content, mineral changes, and mineral distributions. Cross-sectional microhardness testing and light-scattering are also practical indirect techniques for quantitative mineral loss (or gain) determination in intra-oral studies.
本文比较了在口腔内研究中用于评估牙釉质或牙本质脱矿或再矿化的实验技术。在原位研究中，了解有多少矿物质流失或增加以及流失或增加发生的位置非常重要。本文的主要重点是适用于直接或间接矿物质定量的技术。所考虑的测量技术包括显微放射照相术、碘吸收测定法、各种显微硬度测试、偏振光、光散射、碘化物渗透性和湿化学分析。比较了各种技术在以体积百分比（或重量百分比）测定矿物质含量、以体积百分比微米（或千克·平方米）测定矿物质变化以及矿物质分布方面的适用性。此外，还比较并考虑了样品制备、蛋白质渗透的重要性、名义矿物质损失范围、估计的矿物质损失阈值以及对牙本质的适用性。得出的结论是，尽管有十多种技术可用于测量原位脱矿或再矿化后的变化，但横向显微放射照相术是直接和定量测量矿物质含量、矿物质变化及矿物质分布的最实用技术。横断面显微硬度测试和光散射也是口腔内研究中用于定量矿物质损失（或增加）测定的实用间接技术。
