Schwenk R J, Kudo K, Miyamoto M, Sehon A H
J Immunol. 1979 Dec;123(6):2791-8.
Anti-ovalbumin (OA) IgE antibody responses were measured in B6D2F1 mice as a function of time and antigen dose. One hundred to 200 microgram of OA in Al(OH)3 elicited transient responses, whereas 1 to 10 microgram of OA in Al(OH)3 elicited persistent anti-OA IgE responses of high titer. T cells isolated from the spleens of mice mounting either a persistent or a transient response strongly suppressed primary anti-DNP IgE responses in unirradiated recipient mice that were immunized with DNP-OA in Al(OH)3; it was, therefore, concluded that suppressor T cells (Ts cells) were activated during both the persistent and transient IgE responses. Nevertheless, in the present study it was not possible to completely rule out the contention that IgG antibodies may also have been suppressing the IgE response. With a modified adoptive transfer system, it was shown that these Ts cells were sensitive to low doses (250 R) of x-irradiation. The suppressive activity of long-term OA primed cells was also shown to be markedly enhanced when cultured for 24 hr with soluble OA; this finding was interpreted to indicate the presence of memory suppressor cells.
在B6D2F1小鼠中,作为时间和抗原剂量的函数,测量了抗卵清蛋白(OA)IgE抗体反应。100至200微克的OA与氢氧化铝结合引发短暂反应,而1至10微克的OA与氢氧化铝结合则引发高滴度的持续性抗OA IgE反应。从产生持续性或短暂性反应的小鼠脾脏中分离出的T细胞,强烈抑制了未受照射的受体小鼠中初级抗DNP IgE反应,这些受体小鼠用与氢氧化铝结合的DNP-OA进行免疫;因此,得出结论,在持续性和短暂性IgE反应过程中,抑制性T细胞(Ts细胞)均被激活。然而,在本研究中,无法完全排除IgG抗体也可能一直在抑制IgE反应这一论点。使用改良的过继转移系统,结果显示这些Ts细胞对低剂量(250 R)的X射线照射敏感。长期用OA致敏的细胞在与可溶性OA一起培养24小时后,其抑制活性也显著增强;这一发现被解释为表明存在记忆抑制细胞。