不同免疫球蛋白类别的抗体应答调节。II. 小鼠脾细胞体外IgE抗体应答的诱导以及不同T辅助细胞可能参与IgE和IgG抗体应答的证明。

Regulation of antibody response in different immunoglobulin classes. II. Induction of in vitro IgE antibody response in murine spleen cells and demonstration of a possible involvement of distinct T-helper cells in IgE and IgG antibody responses.

作者信息

Kimoto M, Kishimoto T, Noguchi S, Watanabe T, Yamamura Y

出版信息

J Immunol. 1977 Mar;118(3):840-5.

Abstract

In vitro induction of anti-DNP IgE as well as IgG1, IgG2a antibody responses was shown in murine spleen cell culture. Spleen cells primed three times with 1 mug of DNP-OA or DNP-Asc produced significant amounts of anti-DNP IgE as well as IgG antibodies by the in vitro stimulation with DNP-OA or DNP-Asc, respectively. Collaboration between DNP-primed B cells and carrier-primed T cells was required for the induction of both IgE and IgG antibodies with DNP-coupled T-dependent antigen. Carrier-specific T cells induced with a low dose of Asc (0.01 mug) showed helper function only on IgE antibody response, whereas T cells primed with a higher dose of Asc (10 mug) cooperated only with IgG-B cells. T cells primed with Asc in CFA showed helper function mainly on IgG antibody response but not on IgE antibody response. The result indicated the presence of a distinct population of T helper cells for IgE and IgG antibody responses. T-independent antigen (DNP-Ficoll) induced both anti-DNP IgE and IgG antibody responses in DNP-primed spleen cell population without the requirement of the collaboration of helper T cells.

摘要

在小鼠脾细胞培养中显示出抗二硝基苯（DNP）IgE以及IgG1、IgG2a抗体反应的体外诱导。用1μg DNP-卵白蛋白（DNP-OA）或DNP-抗血清（DNP-Asc）致敏三次的脾细胞，分别通过用DNP-OA或DNP-Asc进行体外刺激，产生了大量的抗DNP IgE以及IgG抗体。用DNP偶联的T细胞依赖性抗原诱导IgE和IgG抗体需要DNP致敏的B细胞与载体致敏的T细胞之间的协作。用低剂量的抗血清（0.01μg）诱导的载体特异性T细胞仅对IgE抗体反应显示辅助功能，而用高剂量的抗血清（10μg）致敏的T细胞仅与IgG-B细胞协作。在弗氏完全佐剂（CFA）中用抗血清致敏的T细胞主要对IgG抗体反应显示辅助功能，而对IgE抗体反应无辅助功能。结果表明存在针对IgE和IgG抗体反应的不同群体的辅助性T细胞。T细胞非依赖性抗原（DNP-聚蔗糖）在DNP致敏的脾细胞群体中诱导了抗DNP IgE和IgG抗体反应，而无需辅助性T细胞的协作。