[成人人类睾丸间质细胞的培养与鉴定]
[Culture and identification of human adult Leydig cells].
作者信息
Sun Xiang-Zhou, Deng Chun-Hua, Guo Hai-Bin, Liu Jian-Zhong
机构信息
Department of Urology, First Affiliated Hospital, Sun Yat-sen University, Guangzhou, Guangdong 510080, China.
出版信息
Zhonghua Nan Ke Xue. 2005 May;11(5):356-8.
OBJECTIVE
To obtain a simple and effective method to isolate and purify adult Leydig cells.
METHODS
The testes of human adults were digested and then the density gradient centrifugation of the cells was performed with four different Percoll concentrations (60%, 34%, 26%, 21%) to isolate Leydig cells, whose characteristics were identified by cytological observation staining, 3beta-HSD staining and detection of hCG and testosterone secretion.
RESULTS
High-concentration (> 90%) purified Leydig cells were acquired, and many identification experiments demonstrated the adequate testosterone secretory function of the isolated and purified Leydig cells.
CONCLUSION
This method is easy and efficient for the isolation and purification of adult Leydig cells.
目的
获得一种简单有效的方法来分离和纯化成年睾丸间质细胞。
方法
对成年男性睾丸进行消化处理，然后用四种不同浓度（60%、34%、26%、21%）的Percoll进行细胞密度梯度离心以分离睾丸间质细胞，通过细胞学观察染色、3β-HSD染色以及检测人绒毛膜促性腺激素（hCG）和睾酮分泌来鉴定其特性。
结果
获得了高纯度（>90%）的睾丸间质细胞，多项鉴定实验表明分离纯化的睾丸间质细胞具有充分的睾酮分泌功能。
结论
该方法用于成年睾丸间质细胞的分离和纯化简便且高效。
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