Cao Lixiang, Qiu Zhiqi, You Jianlan, Tan Hongming, Zhou Shining
State Key Laboratory for Biocontrol and Department of Biochemistry, School of Life Sciences, Zhongshan (Sun Yatsen) University, Guangzhou 510275, PR China.
FEMS Microbiol Lett. 2005 Jun 15;247(2):147-52. doi: 10.1016/j.femsle.2005.05.006.
A total of 131 endophytic actinomycete strains were successfully isolated from surface-sterilized banana roots. These isolates belonged to Streptomyces (n=99), Streptoverticillium (n=28), and Streptosporangium (n=2) spp. The remaining 2 isolates were not identified. About 18.3% of the isolates inhibited the growth of pathogenic Fusarium oxysporum f. sp. cubense on banana tissue extract medium. The most frequently isolated Streptomyces sp. strain S96 was similar to Streptomyces griseorubiginosus. About 37.5% of the S. griseorubiginosus strains were antagonistic to F. oxysporum f. sp. cubense. The antagonism of strain S96 was lost when FeCl(3) was introduced into the inhibition zone. In vivo biocontrol assays showed that the disease severity index (DSI) was significantly (P=0.05) reduced and mean fresh weight increased (P=0.001) in plantlets treated with strain S96 compared to those grown in the absence of the biocontrol strain. These findings indicate the potential of developing siderophore-producing Streptomyces endophytes for the biological control of fusarium wilt disease of banana.
从表面消毒的香蕉根中成功分离出131株内生放线菌菌株。这些分离株属于链霉菌属（n = 99）、轮生链霉菌属（n = 28）和孢囊链霉菌属（n = 2）。其余2株未鉴定。约18.3%的分离株在香蕉组织提取物培养基上抑制了致病尖孢镰刀菌古巴专化型的生长。最常分离到的链霉菌属菌株S96与灰红链霉菌相似。约37.5%的灰红链霉菌菌株对尖孢镰刀菌古巴专化型具有拮抗作用。当将FeCl₃引入抑菌圈时，菌株S96的拮抗作用丧失。体内生物防治试验表明，与未接种生防菌株的植株相比，用菌株S96处理的组培苗病情严重指数（DSI）显著降低（P = 0.05），平均鲜重增加（P = 0.001）。这些发现表明开发产生铁载体的链霉菌内生菌用于香蕉枯萎病生物防治的潜力。
