幼年奇努克鲑（Oncorhynchus tshawytscha）胞质谷胱甘肽S-转移酶的特性分析
Characterization of cytosolic glutathione S-transferases in juvenile Chinook salmon (Oncorhynchus tshawytscha).
作者信息
Donham Rachel T, Morin Dexter, Jewell William T, Lamé M W, Segall H J, Tjeerdema Ronald S
机构信息
Department of Environmental Toxicology, College of Agricultural and Environmental Sciences, University of California, 1 Shields Avenue, Davis, CA 95616-8588, USA.
出版信息
Aquat Toxicol. 2005 Jul 1;73(3):221-9. doi: 10.1016/j.aquatox.2005.03.012. Epub 2005 Apr 26.
Four cytosolic glutathione S-transferase (GST) classes were isolated and characterized from juvenile winter run Chinook salmon (Oncorhynchus tshawytscha) liver. Two techniques were used: (1) gel electrophoresis/immunoblotting against a polyclonal striped bass GST antibody and (2) high-pressure liquid chromatography (HPLC). Nanospray liquid chromatography-tandem mass spectrometry (LC-MS/MS) was used to elucidate peptide sequences and the proteins were identified as pi, theta, mu and alpha, by searching against the NCBI non-redundant database (nrDB). Catalytic activity of the cytosolic GSTs towards 1-chloro-2,4-dinitrobenzene (CDNB) and ethacrynic acid (ETHA) were determined to be 0.3+/-0.05 U/mg cytosolic protein and 0.06+/-0.02 U/mg cytosolic protein, respectively.
从幼年冬季洄游型奇努克鲑（Oncorhynchus tshawytscha）肝脏中分离并鉴定了四类胞质谷胱甘肽S-转移酶（GST）。采用了两种技术：（1）针对多克隆条纹鲈GST抗体的凝胶电泳/免疫印迹法，以及（2）高压液相色谱法（HPLC）。采用纳米喷雾液相色谱-串联质谱法（LC-MS/MS）阐明肽序列，并通过在NCBI非冗余数据库（nrDB）中搜索，将这些蛋白质鉴定为pi、theta、mu和alpha。测定胞质GST对1-氯-2,4-二硝基苯（CDNB）和依他尼酸（ETHA）的催化活性分别为0.3±0.05 U/mg胞质蛋白和0.06±0.02 U/mg胞质蛋白。
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