拟南芥WRKY1转录因子C端WRKY结构域的结晶及初步X射线分析
Crystallization and preliminary X-ray analysis of the C-terminal WRKY domain of Arabidopsis thaliana WRKY1 transcription factor.
作者信息
Duan Ming-Rui, Ren Hui, Mao Peng, Wei Chun-Hong, Liang Yu-He, Li Yi, Su Xiao-Dong
机构信息
Peking-Yale Joint Center for Plant Molecular Genetics and Agrobiotechnology, Peking University, Beijing 100871, P.R. China.
出版信息
Biochim Biophys Acta. 2005 Jun 15;1750(1):14-6. doi: 10.1016/j.bbapap.2005.03.013. Epub 2005 Apr 13.
The C-terminal WRKY domain of Arabidopsis thaliana WRKY1 protein, a transcription factor, was cloned and expressed. The expressed protein was then purified and crystallized. The preliminary X-ray analysis was undertaken. The crystal diffracted to 2.50 A resolution in-house and belongs to space group P2(1) with unit-cell parameters a=64.10 A, b=34.88 A, c=114.72 A, beta=90.49 degrees .
拟南芥WRKY1蛋白（一种转录因子）的C端WRKY结构域被克隆并表达。然后对表达的蛋白进行纯化和结晶。进行了初步的X射线分析。该晶体在内部衍射至2.50 Å分辨率，属于空间群P2(1)，晶胞参数为a = 64.10 Å，b = 34.88 Å，c = 114.72 Å，β = 90.49°。
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