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拟南芥WRKY1转录因子C端WRKY结构域的结晶及初步X射线分析

Crystallization and preliminary X-ray analysis of the C-terminal WRKY domain of Arabidopsis thaliana WRKY1 transcription factor.

作者信息

Duan Ming-Rui, Ren Hui, Mao Peng, Wei Chun-Hong, Liang Yu-He, Li Yi, Su Xiao-Dong

机构信息

Peking-Yale Joint Center for Plant Molecular Genetics and Agrobiotechnology, Peking University, Beijing 100871, P.R. China.

出版信息

Biochim Biophys Acta. 2005 Jun 15;1750(1):14-6. doi: 10.1016/j.bbapap.2005.03.013. Epub 2005 Apr 13.

DOI:10.1016/j.bbapap.2005.03.013
PMID:15935985
Abstract

The C-terminal WRKY domain of Arabidopsis thaliana WRKY1 protein, a transcription factor, was cloned and expressed. The expressed protein was then purified and crystallized. The preliminary X-ray analysis was undertaken. The crystal diffracted to 2.50 A resolution in-house and belongs to space group P2(1) with unit-cell parameters a=64.10 A, b=34.88 A, c=114.72 A, beta=90.49 degrees .

摘要

拟南芥WRKY1蛋白(一种转录因子)的C端WRKY结构域被克隆并表达。然后对表达的蛋白进行纯化和结晶。进行了初步的X射线分析。该晶体在内部衍射至2.50 Å分辨率,属于空间群P2(1),晶胞参数为a = 64.10 Å,b = 34.88 Å,c = 114.72 Å,β = 90.49°。

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引用本文的文献

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DNA binding mechanism revealed by high resolution crystal structure of Arabidopsis thaliana WRKY1 protein.拟南芥WRKY1蛋白高分辨率晶体结构揭示的DNA结合机制
Nucleic Acids Res. 2007;35(4):1145-54. doi: 10.1093/nar/gkm001. Epub 2007 Jan 30.