通过小规模丙烯酰胺-CsCl梯度离心法测定DNA碱基组成

Determination of DNA base composition by small scale acrylamide-CsCl gradient centrifugation.

作者信息

Ahn Il-Young, Winter Carlos E

机构信息

Department of Parasitology, Instituto de Ciências Biomédicas, Universidade de São Paulo, Av. Prof. Lineu Prestes, 1374, 05508-900 São Paulo, Brazil.

出版信息

J Biochem Biophys Methods. 2005 Jun 30;63(3):155-60. doi: 10.1016/j.jbbm.2005.03.002.

DOI:10.1016/j.jbbm.2005.03.002

PMID:15936091

Abstract

In this paper we describe a simple and rapid protocol for DNA base composition determination by CsCl gradient in the presence of acrylamide. This method permits the determination of GC content in microgram amounts of DNA, and results are easily documented in photographs or graphs. The protocol was applied to the characterization of nematode DNA, but can be used for other organisms. Analyzing several experiments the mean standard deviation observed in the calculated GC content is near 1.3%.

摘要

在本文中，我们描述了一种在丙烯酰胺存在下通过氯化铯梯度测定DNA碱基组成的简单快速方法。该方法可测定微克量DNA中的GC含量，并且结果易于以照片或图表形式记录。该方法已应用于线虫DNA的表征，但也可用于其他生物体。通过分析多个实验，计算出的GC含量中观察到的平均标准偏差接近1.3%。