Koz'mian L I, Gauze G G, Galkin V I, Dudnik Iu V
Antibiotiki. 1978 Sep;23(9):771-5.
Sibiromycin added to linear chromosomal E. coli DNA in vitro leads to the decrease of bouyant density in neutral CsCl density gradient. This decrease is a linear function of sibiromycin/DNA ratio and amounts to about 32 mg/ml at the ratio equal to 0.1. Binding sibiromycin does not change the degree of hydration of DNA as revealed by centrifugation in metrizamide density gradients. When added to the covalently closed or open circular DNA of PM-2 phage, sibiromycin decreased the bouyant density of these DNA species to a similiar extent. The antibiotic does not induce single-strand breaks in DNA in vitro as follows from the results of ethidium bromide-CsCl density gradient centrifugation of covalently closed PM-2 DNA.
在体外将西伯利亚霉素添加到线性染色体大肠杆菌DNA中会导致其在中性氯化铯密度梯度中的浮力密度降低。这种降低是西伯利亚霉素与DNA比例的线性函数，在比例等于0.1时约为32毫克/毫升。通过在甲泛影酰胺密度梯度中离心发现，结合西伯利亚霉素不会改变DNA的水合程度。当添加到PM-2噬菌体的共价闭合或开放环状DNA中时，西伯利亚霉素会使这些DNA种类的浮力密度降低到类似程度。从共价闭合的PM-2 DNA的溴化乙锭-氯化铯密度梯度离心结果可知，该抗生素在体外不会诱导DNA产生单链断裂。
