Teglas Mike, Matern Erin, Lein Sarah, Foley Patrick, Mahan Suman M, Foley Janet
Center for Vectorborne Diseases, School of Veterinary Medicine, University of California, Davis, CA 95616, USA.
Vet Parasitol. 2005 Jul 15;131(1-2):119-27. doi: 10.1016/j.vetpar.2005.04.033.
Blood samples and ticks were collected from 48 cattle and 74 horses from seven sites in the Peten region of Guatemala. Data on body condition, mucous membrane capillary refill time and tick infestation levels were recorded for each animal in the study. Horses had significantly higher levels of tick infestation than cattle, as well as poorer body condition scores. Seroprevalence of Babesia spp. was 95.8% for B. bovis in cattle, 89.6% for B. bigemina in cattle, and 92.7% for B. equi in horses. Seroprevalence of Anaplasma marginale in cattle was 87.5%, similar to reports in animals from other regions of Central America. This is the first time that A. phagocytophilum has been reported in animals from this region, with overall PCR-prevalence of 27.6% in cattle and horses, and seroprevalence of 28.4% (52% in cattle and 13% in horses). An agent was identified with serological cross-reactivity and close genetic relatedness to Ehrlichia ruminantium, but further testing confirmed that the agent in Guatemalan cows was not the agent of heartwater. Ticks were identified to species with the predominant species identified on cattle as Boophilus microplus and Amblyomma cajennense, while Anocentor nitens and A. cajennense were most commonly found on horses. Prevalence of infection, tick infestation levels, host factors and environmental data were analyzed for association; A. nitens was significantly associated with A. phagocytophilum prevalence by village.
从危地马拉佩滕地区的七个地点的48头牛和74匹马身上采集了血样和蜱虫。记录了研究中每只动物的身体状况、粘膜毛细血管再充盈时间和蜱虫感染水平的数据。马的蜱虫感染水平明显高于牛，身体状况评分也较差。牛的牛巴贝斯虫血清阳性率为95.8%，双芽巴贝斯虫为89.6%，马的马巴贝斯虫为92.7%。牛的边缘无形体血清阳性率为87.5%，与中美洲其他地区动物的报道相似。这是该地区动物首次报告嗜吞噬细胞无形体，牛和马的总体PCR阳性率为27.6%，血清阳性率为28.4%（牛为52%，马为13%）。鉴定出一种与反刍动物埃立克体具有血清学交叉反应和密切遗传关系的病原体，但进一步检测证实危地马拉奶牛中的病原体不是心水病的病原体。蜱虫被鉴定到种，牛身上的主要蜱种为微小牛蜱和卡延花蜱，而马身上最常见的是嗜皮血蜱和卡延花蜱。分析了感染率、蜱虫感染水平、宿主因素和环境数据之间的关联；按村庄划分，嗜皮血蜱与嗜吞噬细胞无形体的流行率显著相关。
