通过酶联免疫斑点法（ELISPOT）检测结核病动物模型中的T细胞功能。

Measuring T-cell function in animal models of tuberculosis by ELISPOT.

作者信息

Lazarevic Vanja, Pawar Santosh, Flynn Joanne

机构信息

Dept. of Molecular Genetics and Biochemistry, University of Pittsburgh, Pittsburgh, PA, USA.

出版信息

Methods Mol Biol. 2005;302:179-90. doi: 10.1385/1-59259-903-6:179.

DOI:10.1385/1-59259-903-6:179

PMID:15937352

Abstract

Enzyme-linked immunospot (ELISPOT) was originally developed from an enzyme-linked immunosorbent assay (ELISA) to detect and measure the frequency of individual cells that produce cytokines in response to antigenic stimulation. ELISPOT assay is more sensitive than the enzyme-linked immunosorbent assay or intracellular cytokine staining. Increased sensitivity of ELISPOT is particularly advantageous when studying T-cell-mediated responses in Mycobacterium tuberculosis infection because antigen specific T-cells can occur at a low frequency in vivo. This method has been successfully used to analyze M. tuberculosis immune responses in humans in addition to murine and nonhuman primate models of tuberculosis.

摘要

酶联免疫斑点法（ELISPOT）最初是从酶联免疫吸附测定法（ELISA）发展而来的，用于检测和测量因抗原刺激而产生细胞因子的单个细胞的频率。ELISPOT测定法比酶联免疫吸附测定法或细胞内细胞因子染色更敏感。当研究结核分枝杆菌感染中T细胞介导的反应时，ELISPOT的更高敏感性特别有利，因为抗原特异性T细胞在体内可能以低频率出现。除了结核病的小鼠和非人类灵长类动物模型外，该方法已成功用于分析人类的结核分枝杆菌免疫反应。