Gazagne Agnes, Malkusch Wolf, Vingert Benoit, Fridman Wolf H, Tartour Eric
Unité d'Immunologie Biologique, Hôpital Européen Georges Pompidou, Université René Descartes, Paris, France.
Methods Mol Biol. 2005;302:289-96. doi: 10.1385/1-59259-903-6:289.
The conventional enzyme-linked immunospot (ELISPOT) technique detects only one secreted cytokine, which constitutes a major pitfall for the accurate characterization of the various T-cell subpopulations. We have therefore developed a fluorospot assay, which is a modification of the ELISPOT and is based on the use of multiple fluorescent-labeled anticytokines detection antibodies. A special automated ELISPOT reader consisting of a light microscope with incident fluorescence illumination and an integrating digital color camera has been adapted for this technique. This technique has been applied for the analysis of subpopulations of T-cells and polarized antigen-specific T-cells.
传统的酶联免疫斑点（ELISPOT）技术仅能检测一种分泌的细胞因子，这对于准确鉴定各种T细胞亚群而言是一个主要缺陷。因此，我们开发了一种荧光斑点分析方法，它是对ELISPOT的改进，基于使用多种荧光标记的抗细胞因子检测抗体。一种特殊的自动ELISPOT读数仪，由一台带有落射荧光照明的光学显微镜和一台积分式数字彩色相机组成，已被适配用于该技术。该技术已应用于T细胞亚群和极化抗原特异性T细胞的分析。
