Takami H, Naramoto A, Shigematsu H, Ohno S
Second Department of Medicine, Tokyo Medical and Dental University, Japan.
Nihon Jinzo Gakkai Shi. 1992 Jan;34(1):41-6.
Rat kidney tissue was quickly frozen, fractured and deeply etched. A replica was prepared by shadowing with platinum and carbon. The proximal tubular basement membrane was found to be different from that in conventional ultrathin sections. The lamina lucida was not electron translucent but filled with a traversing filamentous structure, which connected the tubular cell membranes with the lamina densa. The lamina densa had a three-dimensional polygonal meshwork structure. This meshwork was composed of fibrils (6-9 nm in thickness). In the lamina reticularis, collagen fibrils and micro-threads formed a loose network structure. The traversing filamentous structure in the lamina lucida might serve as an anchorage for the cell membranes and extracellular matrices.
大鼠肾脏组织迅速冷冻、断裂并深度蚀刻。通过用铂和碳进行投影制备复制品。发现近端肾小管基底膜与传统超薄切片中的不同。透明层并非电子透明，而是充满了横向丝状结构，该结构将肾小管细胞膜与致密层相连。致密层具有三维多边形网状结构。该网状结构由原纤维（厚度为6 - 9纳米）组成。在网状层中，胶原原纤维和微丝形成松散的网络结构。透明层中的横向丝状结构可能作为细胞膜和细胞外基质的锚定物。
