Gu Deng-an, Li Ai-ping, Zhu Ting, Ye Jian, Zhou Jian-wei
Department of Molecular Cell Biology and Toxicology, Jiangsu Provincial Key Laboratories of Human Functional Genomics and of Toxicology, Nanjing Medical University, Nanjing 210029, China.
Zhonghua Yu Fang Yi Xue Za Zhi. 2005 May;39(3):187-90.
To investigate the expressions of JWA gene and heat shock protein 70 (hsp70) in human embryonic lung (cccHPF-1) cells after exposure to activated benzo(a)pyrene (B(a)P), and to explore the role and the possible mechanism of JWA gene involved in B(a)P-induced DNA damage and repair.
The models of DNA damage of ccc-HPF-1 cells were established by treatment of cells with B(a)P plus S9 at 10 to 100 micromol/L for 3 hours with or without 24 hours recovery for DNA repairing. The DNA damage was detected by single cell gel electrophoresis (SCGE) assay (comet assay). And the immuno-blotting assay was used for detecting expressions of JWA and hsp70.
JWA expression was actively modulated by B(a)P exposure. The expressions of both JWA and hsp70 were increased greatly at 50 micromol/L and 100 micromol/L B(a)P treated cells and maintained at over expressed levels treated by 10-100 micromol/L B(a)P during the restored time. In addition, JWA expression pattern was similar to that of hsp70.
JWA is determined in this study by its functioning as an effective environmental responsive gene and actively participating in the signal pathways of DNA damage and repair which might be associated with excision repair.
研究人胚肺（cccHPF-1）细胞在暴露于活化苯并(a)芘（B(a)P）后JWA基因和热休克蛋白70（hsp70）的表达情况，探讨JWA基因在B(a)P诱导的DNA损伤及修复中的作用和可能机制。
用10至100微摩尔/升的B(a)P加S9处理细胞3小时，建立ccc-HPF-1细胞DNA损伤模型，DNA损伤修复时有无24小时恢复。通过单细胞凝胶电泳（SCGE）试验（彗星试验）检测DNA损伤。采用免疫印迹试验检测JWA和hsp70的表达。
B(a)P暴露可积极调节JWA表达。在50微摩尔/升和100微摩尔/升B(a)P处理的细胞中，JWA和hsp70的表达均显著增加，并在10至100微摩尔/升B(a)P处理的恢复时间内维持在过表达水平。此外，JWA的表达模式与hsp70相似。
本研究确定JWA作为一种有效的环境反应基因发挥作用，并积极参与可能与切除修复相关的DNA损伤和修复信号通路。
