Song S K, Hotchkiss R S, Karl I E, Ackerman J J
Department of Chemistry, Washington University, St. Louis, Missouri 63130-4899.
Magn Reson Med. 1992 May;25(1):67-77. doi: 10.1002/mrm.1910250107.
In the conclusion of this series of reports, the application of 31P/2H NMR to investigate the pathophysiology of sepsis in rat hindlimb muscle is demonstrated. Sepsis decreased muscle [PCr] by 18%, 18 +/- 4 SD vs 22 +/- 4 SD mmol/kg tissue wet wt (P = 0.01) in control rats but [ATP] was unchanged, 6 mmol/kg tissue wet wt (P = 0.2). The derived free cytosolic [ADP] in the two groups was similar, [ADP]septic = 0.023 +/- 0.004 SD and [ADP]control = 0.021 +/- 0.003 SD mmol/kg tissue wet wt, and not statistically different (P = 0.14). Likewise [Pi] in the septic and control groups was not statistically different, [Pi]septic = 1.1 +/- 0.5 SD and [Pi]control = 1.2 +/- 0.4 SD mmol/kg tissue wet wt (P = 0.2). Septic rats presented the symptom of respiratory alkalosis evidenced by elevated blood pH. Sepsis decreased muscle blood flow by 33%, P = 0.003, but examination of individual subjects did not demonstrate a correlation with the reduction in [PCr]. Thus, a metabolic energy deficit caused by cellular ischemia/hypoxia is not a likely cause of cellular abnormality in rat hindlimb muscle during sepsis.
在这一系列报告的结论中，展示了应用31P/2H NMR研究大鼠后肢肌肉脓毒症病理生理学的情况。脓毒症使肌肉中的磷酸肌酸（[PCr]）降低了18%，脓毒症大鼠为18±4 SD mmol/kg组织湿重，而对照大鼠为22±4 SD mmol/kg组织湿重（P = 0.01），但三磷酸腺苷（[ATP]）未改变，均为6 mmol/kg组织湿重（P = 0.2）。两组中计算得出的游离胞质二磷酸腺苷（[ADP]）相似，脓毒症组[ADP]septic = 0.023±0.004 SD，对照组[ADP]control = 0.021±0.003 SD mmol/kg组织湿重，差异无统计学意义（P = 0.14）。同样，脓毒症组和对照组中的无机磷酸盐（[Pi]）差异也无统计学意义，脓毒症组[Pi]septic = 1.1±0.5 SD，对照组[Pi]control = 1.2±0.4 SD mmol/kg组织湿重（P = 0.2）。脓毒症大鼠出现呼吸性碱中毒症状，表现为血液pH值升高。脓毒症使肌肉血流量减少了33%，P = 0.003，但对个体受试者的检查未显示与[PCr]降低存在相关性。因此，细胞缺血/缺氧导致的代谢能量缺乏不太可能是脓毒症期间大鼠后肢肌肉细胞异常的原因。
