体外强大的丙型肝炎病毒感染
Robust hepatitis C virus infection in vitro.
作者信息
Zhong Jin, Gastaminza Pablo, Cheng Guofeng, Kapadia Sharookh, Kato Takanobu, Burton Dennis R, Wieland Stefan F, Uprichard Susan L, Wakita Takaji, Chisari Francis V
机构信息
Departments of Molecular and Experimental Medicine and Immunology, The Scripps Research Institute, La Jolla, CA 92037, USA.
出版信息
Proc Natl Acad Sci U S A. 2005 Jun 28;102(26):9294-9. doi: 10.1073/pnas.0503596102. Epub 2005 Jun 6.
Abstract
The absence of a robust cell culture model of hepatitis C virus (HCV) infection has severely limited analysis of the HCV life cycle and the development of effective antivirals and vaccines. Here we report the establishment of a simple yet robust HCV cell culture infection system based on the HCV JFH-1 molecular clone and Huh-7-derived cell lines that allows the production of virus that can be efficiently propagated in tissue culture. This system provides a powerful tool for the analysis of host-virus interactions that should facilitate the discovery of antiviral drugs and vaccines for this important human pathogen.
摘要
丙型肝炎病毒（HCV）感染缺乏强大的细胞培养模型，这严重限制了对HCV生命周期的分析以及有效抗病毒药物和疫苗的研发。在此，我们报告基于HCV JFH - 1分子克隆和源自Huh - 7的细胞系建立了一种简单而强大的HCV细胞培养感染系统，该系统能够产生可在组织培养中有效传代的病毒。该系统为分析宿主 - 病毒相互作用提供了一个强大的工具，应有助于发现针对这种重要人类病原体的抗病毒药物和疫苗。
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