斯坦尼斯拉斯队列中CYP2A6*1B基因变异与法国成年人吸烟量的关联

Association of CYP2A6*1B genetic variant with the amount of smoking in French adults from the Stanislas cohort.

作者信息

Gambier N, Batt A-M, Marie B, Pfister M, Siest G, Visvikis-Siest S

机构信息

Inserm U525, Faculté de Pharmacie, Université Henri Poincaré-Nancy1, Nancy, France.

出版信息

Pharmacogenomics J. 2005;5(4):271-5. doi: 10.1038/sj.tpj.6500314.

DOI:10.1038/sj.tpj.6500314

PMID:15940289

Abstract

This study was designed in order to investigate the influence of the genetic polymorphism of CYP2A6 on the amount of smoking. In all, 463 French adults included in the Stanislas cohort were studied and underwent two examinations at 5 years distance (t0 and t(+5) years). Information on their smoking habits was collected. They were genotyped by RFLP for the CYP2A61A, CYP2A61B and CYP2A64 alleles. CYP2A61B and CYP2A64 allele frequencies were 32 and 4%, respectively. The subjects carrying the CYP2A61B allele oxidize nicotine to cotinine faster than subjects with the CYP2A61A allele. The number of cigarettes smoked per day was significantly higher in the CYP2A61B/1B group as compared to the CYP2A61A/1A group (P = 0.01 at t0; P = 0.001 t(+5) years), with a larger increase in their daily cigarettes consumption over the 5-year period (P = 0.006). No significant difference in the smoking status was observed according to the CYP2A6 genotype. These data suggest that the CYP2A61B is associated with the number of cigarettes smoked per day.

摘要

本研究旨在调查CYP2A6基因多态性对吸烟量的影响。总共对斯坦尼斯拉斯队列中的463名法国成年人进行了研究，并在5年的间隔时间内（t0和t（+5）年）进行了两次检查。收集了他们的吸烟习惯信息。通过限制性片段长度多态性（RFLP）对他们进行CYP2A61A、CYP2A61B和CYP2A64等位基因的基因分型。CYP2A61B和CYP2A64等位基因频率分别为32%和4%。携带CYP2A61B等位基因的受试者将尼古丁氧化为可替宁的速度比携带CYP2A61A等位基因的受试者更快。与CYP2A61A/1A组相比，CYP2A61B/1B组每天吸烟的数量显著更高（t0时P = 0.01；t（+5）年时P = 0.001），且在5年期间其每日吸烟量的增加幅度更大（P = 0.006）。根据CYP2A6基因型，未观察到吸烟状况的显著差异。这些数据表明，CYP2A61B与每天吸烟的数量有关。