Yamauchi Daisuke, Sutoh Keita, Kanegae Hiromi, Horiguchi Tatsuya, Matsuoka Ken, Fukuda Hiroo, Wada Masamitsu
Department of Life Science, University of Hyogo, Himeji, Japan.
J Plant Res. 2005 Jun;118(3):223-7. doi: 10.1007/s10265-005-0209-3. Epub 2005 Jun 7.
The analysis of expressed sequences from a diverse set of plant species has fueled the increase in understanding of the complex molecular mechanisms underlying plant growth regulation. While representative data sets can be found for the major branches of plant evolution, fern species data are lacking. To further the availability of genetic information in pteridophytes, a normalized cDNA library of Adiantum capillus-veneris was constructed from prothallia grown under white light. A total of 10,420 expressed sequence tags (ESTs) were obtained and clustering of these sequences resulted in 7,100 nonredundant clusters. Of these, 1,608 EST clusters were found to be similar to sequences of known function and 1,092 EST clusters showed similarity to sequences of unknown function. Given the usefulness of Adiantum for developmental studies, the sequence data represented in this report stand to make a significant contribution to the understanding of plant growth regulation, particularly for pteridophytes.
对多种植物物种表达序列的分析，推动了人们对植物生长调控背后复杂分子机制的理解。虽然在植物进化的主要分支中可以找到代表性数据集，但蕨类植物的数据却很缺乏。为了进一步增加蕨类植物的遗传信息，从在白光下生长的原叶体构建了一个扇叶铁线蕨的标准化cDNA文库。共获得了10420个表达序列标签（EST），对这些序列进行聚类后得到7100个非冗余簇。其中，发现1608个EST簇与已知功能的序列相似，1092个EST簇与功能未知的序列相似。鉴于铁线蕨在发育研究中的有用性，本报告中的序列数据有望为理解植物生长调控，特别是蕨类植物的生长调控做出重大贡献。
