Jiang Qing-wu, Li Qin-xue, Chen Zheng, Shen Jie, Zhong Pei-song, Zhang Xin, Tan Jia-di, Shao Yue-qin, Li Zi-hua
School of Public Health, Fudan University, Shanghai 200032, China.
Zhonghua Liu Xing Bing Xue Za Zhi. 2005 Mar;26(3):204-6.
To study the survival and growth of Vibrio cholerae inside the Acanthamoeba polyphage.
Survival and growth of Vibro cholerae O139, co-cultured with Acanthamoeba polyphaga, was observed inside the trophozoites and cysts, using Gram stain and electron microscope.
Viable O139 was observed inside the amoebal vacuoles in 24 hours. Vacuoles were filled with more bacteria along with the longer period of co-culture. The process of O139 infection with Amoebae would include uptake, formation of O139 vacuole, multiplication, trophozoites lysed and expel under electron microscopy. Some infected trophozoites could subsequently encyst and the surviving O139 could locate in the vesicles inside the cysts.
O139 might survive and multiply in the trophozoites and reside inside the cysts of Amoebae, suggesting that Acanthamoebae might serve as one of the environmental hosts of Vibro cholerae.
研究霍乱弧菌在多噬棘阿米巴体内的存活及生长情况。
采用革兰氏染色和电子显微镜观察，研究与多噬棘阿米巴共培养的O139霍乱弧菌在滋养体和包囊内的存活及生长情况。
24小时内可在阿米巴泡内观察到有活力的O139。随着共培养时间延长，泡内充满了更多细菌。在电子显微镜下，O139感染阿米巴的过程包括摄取、O139泡的形成、增殖、滋养体裂解并排出。一些受感染的滋养体随后可形成包囊，存活的O139可位于包囊内的小泡中。
O139可能在滋养体内存活并增殖，并存在于阿米巴的包囊内，提示棘阿米巴可能是霍乱弧菌的环境宿主之一。
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