Abd Hadi, Saeed Amir, Weintraub Andrej, Sandström Gunnar
Centre for Microbiological Preparedness, Swedish Institute for Infectious Disease Control, SE-171 82 Solna, Sweden.
Division of Clinical Microbiology, Department of Laboratory Medicine, Karolinska Institute, Karolinska University Hospital Huddinge, SE-14186 Stockholm, Sweden.
J Med Microbiol. 2009 Jan;58(Pt 1):125-131. doi: 10.1099/jmm.0.004721-0.
Vibrio cholerae, the causative agent of cholera, has the ability to grow and survive in the aquatic free-living amoeba Acanthamoeba castellanii. The aim of the present study was to examine the ability of the clinical isolate V. cholerae O139 MO10 to grow in A. castellanii and to determine the effect of the bacterial capsule and LPS O side chain on intracellular growth. Results from co-cultivation, viable counts, a gentamicin assay, electron microscopy and statistical analysis showed that the association of V. cholerae O139 MO10 with A. castellanii did not inhibit growth of the amoeba, and enhanced growth and survival of V. cholerae O139 MO10 occurred. The wild-type V. cholerae O139 MO10 and a capsule mutant or capsule/LPS double mutant grew inside A. castellanii. Neither the capsule nor the LPS O side chain of V. cholerae O139 was found to play an important role in the interaction with A. castellanii, disclosing the ability of V. cholerae to multiply and survive inside A. castellanii, as well as the role of A. castellanii as an environmental host for V. cholerae.
霍乱弧菌是霍乱的病原体，具有在水生自由生活的变形虫——卡氏棘阿米巴中生长和存活的能力。本研究的目的是检测临床分离株霍乱弧菌O139 MO10在卡氏棘阿米巴中的生长能力，并确定细菌荚膜和脂多糖O侧链对细胞内生长的影响。共培养、活菌计数、庆大霉素测定、电子显微镜检查和统计分析结果表明，霍乱弧菌O139 MO10与卡氏棘阿米巴的结合并未抑制变形虫的生长，且霍乱弧菌O139 MO10的生长和存活得到了增强。野生型霍乱弧菌O139 MO10以及荚膜突变体或荚膜/脂多糖双突变体在卡氏棘阿米巴内部生长。未发现霍乱弧菌O139的荚膜和脂多糖O侧链在与卡氏棘阿米巴的相互作用中起重要作用，这揭示了霍乱弧菌在卡氏棘阿米巴内部繁殖和存活的能力，以及卡氏棘阿米巴作为霍乱弧菌环境宿主的作用。
