Zhang Li-jun, Xiong Hong-yan, Zhang Yao, Ma Fei, Li Li
Department of Military Epidemiology, Academy of Preventive Medicine, The Third Military Medical University, Chongqing 400038, China.
Zhonghua Liu Xing Bing Xue Za Zhi. 2005 Mar;26(3):207-10.
To explore the germination effects of Bacillus anthracoides spores germinant to nutrient germinant.
Heat factors and nutrient germinant were used to stimulate the Bacillus anthracoides spores and to germinate. Ultraviolet spectrophotometer was used to measured the A value of spore solution in the wavelength of 600 nm. Accrding to the A value, the germination rates in different condition. Transmission electron microscope was used to observe the ultrastructure changes of spores.
The rate of germination effects were 68.0% under 6 mmol/L inosine at 37 degrees C, pH 7.9; 74.5% under 70 mmol/L L-alanine at 30 degrees C, pH 8.9; and 85.6% under 6 mmol/L inosine and 70 mmol/L L-alanine at 37 degrees C, pH 8.2. Under transmission electron microscope, the germinated spores' coat and cortex were brokendown and degraded with its core completely exposed.
Under suitable environment, the nutrient germinant with inosine and L-alanine might be helpful for germinating the bacillus anthracoides spores.
探讨炭疽芽孢杆菌芽孢对营养萌发剂的萌发效果。
利用热因素和营养萌发剂刺激炭疽芽孢杆菌芽孢并使其萌发。采用紫外分光光度计测定芽孢悬液在600nm波长处的吸光度（A值）。根据A值计算不同条件下的萌发率。用透射电子显微镜观察芽孢的超微结构变化。
在37℃、pH 7.9条件下，6mmol/L肌苷作用时萌发率为68.0%；在30℃、pH 8.9条件下，70mmol/L L-丙氨酸作用时萌发率为74.5%；在37℃、pH 8.2条件下，6mmol/L肌苷和70mmol/L L-丙氨酸共同作用时萌发率为85.6%。透射电子显微镜下可见，萌发后的芽孢外壳和皮层破裂降解，核心完全暴露。
在适宜环境下，含肌苷和L-丙氨酸的营养萌发剂可能有助于炭疽芽孢杆菌芽孢的萌发。
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