Gounina-Allouane Rabia, Broussolle Véronique, Carlin Frédéric
UMR 408 Sécurité et Qualité des Produits d'Origine Végétale, INRA, Université d'Avignon, Site Agroparc, F-84000 Avignon, Cedex, France.
Food Microbiol. 2008 Feb;25(1):202-6. doi: 10.1016/j.fm.2007.08.002. Epub 2007 Sep 3.
The effect of sporulation temperature on Bacillus cereus spore germination triggered by the nutrient germinants inosine and l-alanine was investigated. The germination (expressed as % A(600nm) fall) of heat-activated spores of B. cereus strain ATCC14579 produced at 20 and 37 degrees C, and of psychrotrophic strains LM9 and D15 produced at 15 and 37 degrees C was followed in a germination buffer containing inosine at concentrations between 0.01 and 10mmoll(-1), or l-alanine between 1 and 100mmoll(-1). Spores wet-heat resistance at 90 degrees C was also determined. Spores of the three strains produced at the lowest temperatures generally showed a higher germination capacity in response to both inosine and in l-alanine than those produced at 37 degrees C, and were also more susceptible to heat. Low sporulation temperature is confirmed as a detrimental factor to B. cereus spore wet-heat resistance, and conversely gave spores with a sensitivity to the nutrient germinants inosine and l-alanine higher than that of spores formed close to the maximal sporulation temperature.
研究了芽孢形成温度对蜡样芽孢杆菌孢子萌发的影响，该萌发由营养萌发剂肌苷和L-丙氨酸触发。在含有浓度介于0.01至10 mmol l⁻¹之间的肌苷，或1至100 mmol l⁻¹之间的L-丙氨酸的萌发缓冲液中，跟踪测定在20和37摄氏度产生的蜡样芽孢杆菌ATCC14579菌株的热激活孢子，以及在15和37摄氏度产生的嗜冷菌株LM9和D15的孢子萌发情况（以A(600nm)下降百分比表示）。还测定了三种菌株的孢子在90摄氏度下的湿热抗性。在最低温度下产生的三种菌株的孢子，通常对肌苷和L-丙氨酸的萌发能力均高于在37摄氏度下产生的孢子，并且对热也更敏感。低芽孢形成温度被确认为蜡样芽孢杆菌孢子湿热抗性的不利因素，相反，与在接近最大芽孢形成温度下形成的孢子相比，低芽孢形成温度下产生的孢子对营养萌发剂肌苷和L-丙氨酸更为敏感。
