Serrano-Heras Gemma, Salas Margarita, Bravo Alicia
Instituto de Biología Molecular Eladio Viñuela (CSIC), Centro de Biología Molecular Severo Ochoa (CSIC-UAM), Universidad Autónoma, Cantoblanco, 28049 Madrid, Spain.
Plasmid. 2005 Nov;54(3):278-82. doi: 10.1016/j.plasmid.2005.04.002. Epub 2005 Jun 6.
We have developed a novel regulated expression vector for Bacillus subtilis based on the Staphylococcus aureus plasmid pUB110. This vector, named pPR54, carries the P(R) promoter and the cI857 gene (encoding a temperature-sensitive transcriptional repressor) from the Escherichia coli phage lambda. Using the gfp gene from the jellyfish Aequorea victoria as a reporter, we show that pPR54 is a useful vector for controllable production of heterologous proteins in B. subtilis.
我们基于金黄色葡萄球菌质粒pUB110开发了一种用于枯草芽孢杆菌的新型调控表达载体。该载体名为pPR54，携带来自大肠杆菌噬菌体λ的P(R)启动子和cI857基因（编码一种温度敏感型转录阻遏物）。使用来自维多利亚多管水母的gfp基因作为报告基因，我们证明pPR54是一种用于在枯草芽孢杆菌中可控生产异源蛋白的有用载体。
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