Kovacs S H
In Vitro. 1977 Jan;13(1):24-30. doi: 10.1007/BF02615500.
Primary cultures of 10-day embryonic chick neural retinas were used to investigate early aspects of the mechanism of hydrocortisone action on glutamine synthetase activity. As little as 2 hr of hydrocortisone exposure served to initiate significant increases in the glutamine synthetase activity levels assayed after 24 hr culture. Time course studies indicated that the increase in glutamine synthetase activity observed after 24 hr in culture resulted from a two-phase rise in activity and that cycloheximide was effective in suppressing the second-phase rise. Additional inhibition studies demonstrated that the second-phase increase in enzyme activity required continuous protein synthesis during the initial 6 hr. The evidence suggests a mechanism of hydrocortisone action involving the production of a protein which is important for the induction of glutamine synthetase activity by hydrocortisone.
利用10日龄鸡胚神经视网膜的原代培养物来研究氢化可的松对谷氨酰胺合成酶活性作用机制的早期方面。仅暴露于氢化可的松2小时就足以在培养24小时后测定的谷氨酰胺合成酶活性水平上引发显著增加。时间进程研究表明，培养24小时后观察到的谷氨酰胺合成酶活性增加是由活性的两阶段上升引起的，并且环己酰亚胺可有效抑制第二阶段的上升。额外的抑制研究表明，酶活性的第二阶段增加在最初6小时需要持续的蛋白质合成。证据表明氢化可的松的作用机制涉及一种蛋白质的产生，该蛋白质对于氢化可的松诱导谷氨酰胺合成酶活性很重要。
