干扰素可抑制胚胎期鸡神经视网膜中类固醇诱导的谷氨酰胺合成酶的生物合成。

Interferon suppresses steroid-inducible glutamine synthetase biosynthesis in embryonic chick neural retina.

作者信息

Matsuno T, Shirasawa N

出版信息

Biochim Biophys Acta. 1978 Jan 18;538(2):188-94. doi: 10.1016/0304-4165(78)90345-8.

Abstract

Effect of chick interferon on the biosynthesis of glutamine synthetase (L-glutamate: ammonia ligase (ADP-forming), EC 6.3.1.2) was studied in the embryonic chick neural retina cultures induced for the enzyme activity by hydrocortisone. The retinal enzyme radioactively labelled with [3H]leucine was precipitated by specific antibody against the enzyme isolated from adult chick liver. The immunological determination offered evidence that the suppressive effect of interferon on the hormonal induction of the enzyme was primarily due to reduced rate of its synthesis and accumulation.

摘要

在由氢化可的松诱导产生谷氨酰胺合成酶（L-谷氨酸：氨连接酶（ADP形成），EC 6.3.1.2）活性的鸡胚神经视网膜培养物中，研究了鸡干扰素对该酶生物合成的影响。用[3H]亮氨酸进行放射性标记的视网膜酶，可被针对从成年鸡肝脏分离出的该酶的特异性抗体沉淀。免疫测定提供的证据表明，干扰素对该酶激素诱导的抑制作用主要是由于其合成和积累速率降低。