通过二维凝胶电泳和表面增强激光解吸电离飞行时间质谱法鉴定结直肠癌组织中的蛋白质。

Identification of proteins from colorectal cancer tissue by two-dimensional gel electrophoresis and SELDI mass spectrometry.

作者信息

Melle Christian, Osterloh Dirk, Ernst Günther, Schimmel Bettina, Bleul Annett, von Eggeling Ferdinand

机构信息

Core Unit Chip Application, Institute of Human Genetics and Anthropology, Friedrich-Schiller University, Jena 07740, Germany.

出版信息

Int J Mol Med. 2005 Jul;16(1):11-7.

PMID:15942672

Abstract

We investigated protein profiles obtained from colorectal tumor tissue and adjacent normal mucosa to identify tumor specific changes. Protein extracts of biopsis were separated by two-dimensional gel electrophoresis and >40 low-molecular mass proteins were identified by peptide fingerprinting using surface-enhanced laser desoption/ionization mass spectrometry (SELDI-MS). Among these, PACAP protein, hnrnp A1, flavin reductase, calgizzarin, NDK B (NM23-H2), cyclophilin A and smooth muscle protein 22-alpha showed significantly differential abundancy in the analyzed specimens. In addition, immunohistochemical analysis of tissue distribution and subcellular localization of some of the differentially expressed proteins demonstrated alterations in subcellular protein distribution. Further investigations are in progress to assess whether these differentially expressed proteins are associated with tumor development and tumor progression.

摘要

我们研究了从结直肠肿瘤组织和相邻正常黏膜获得的蛋白质谱，以识别肿瘤特异性变化。活检组织的蛋白质提取物通过二维凝胶电泳进行分离，使用表面增强激光解吸/电离质谱（SELDI-MS）通过肽指纹图谱鉴定了40多种低分子量蛋白质。其中，PACAP蛋白、hnrnp A1、黄素还原酶、钙结合蛋白、NDK B（NM23-H2）、亲环蛋白A和平滑肌蛋白22-α在分析的标本中显示出明显的丰度差异。此外，对一些差异表达蛋白质的组织分布和亚细胞定位进行的免疫组织化学分析表明亚细胞蛋白质分布发生了改变。正在进行进一步的研究，以评估这些差异表达的蛋白质是否与肿瘤发生和肿瘤进展相关。