hnRNP A1、Ezrin、微管蛋白 β-2C 和膜联蛋白 A1 在结直肠癌前哨淋巴结中的上调。
Up-regulation of hnRNP A1, Ezrin, tubulin β-2C and Annexin A1 in sentinel lymph nodes of colorectal cancer.
机构信息
Department of General Surgery, Second Affiliated Hospital of Nanjing Medical University, 121 Jiangjiayuan Road, Nanjing 210011, Jiangsu Province, China.
出版信息
World J Gastroenterol. 2010 Oct 7;16(37):4670-6. doi: 10.3748/wjg.v16.i37.4670.
AIM
To investigate the early metastasis-associated proteins in sentinel lymph node micrometastasis (SLNMM) of colorectal cancer (CRC) through comparative proteome.
METHODS
Hydrophobic protein samples were extracted from individual-matched normal lymph nodes (NLN) and SLNMM of CRC. Differentially expressed protein spots were detected by two-dimensional electrophoresis and image analysis, and subsequently identified by matrix assisted laser desorption/ionization-time of flight mass spectrometry-mass spectrometry and Western blotting, respectively.
RESULTS
Forty proteins were differentially expressed in NLN and SLNMM, and 4 metastasis-concerned proteins highly expressed in SLNMM were identified to be hnRNP A1, Ezrin, tubulin β-2C and Annexin A1. Further immunohistochemistry staining of these four proteins showed their clinicopathological characteristics in lymph node metastasis of CRC.
CONCLUSION
Variations of hydrophobic protein expression in NLN and SLNMM of CRC and increased expression of hnRNP A1, Ezrin, tubulin β-2C and Annexin A1 in SLNMM suggest a significantly elevated early CRC metastasis.
目的
通过比较蛋白质组学方法研究结直肠癌（CRC）前哨淋巴结微转移（SLNMM）中早期转移相关蛋白。
方法
从个体匹配的正常淋巴结（NLN）和 CRC 的 SLNMM 中提取疏水性蛋白质样品。通过二维电泳和图像分析检测差异表达的蛋白质斑点，然后分别通过基质辅助激光解吸/电离飞行时间质谱-质谱和 Western 印迹进行鉴定。
结果
NLN 和 SLNMM 中表达差异的蛋白质有 40 种，在 SLNMM 中高表达的 4 种转移相关蛋白被鉴定为 hnRNP A1、Ezrin、微管蛋白β-2C 和膜联蛋白 A1。对这四种蛋白质的进一步免疫组织化学染色显示了它们在 CRC 淋巴结转移中的临床病理特征。
结论
CRC 的 NLN 和 SLNMM 中疏水性蛋白表达的变化以及 hnRNP A1、Ezrin、微管蛋白β-2C 和膜联蛋白 A1 在 SLNMM 中的表达增加提示 CRC 早期转移明显升高。
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