Katoh Yuriko, Katoh Masaru
M&M Medical BioInformatics, Hongo 113-0033, Japan.
Oncol Rep. 2005 Jul;14(1):291-4.
Human FGF19 is the ortholog of rodent Fgf15, and 22 FGF family genes exist within the human genome as well as within the rodent genome. Mouse Fgf11 refSeq NM_010198.1 and rat Fgf11 RefSeq NM_130816.1 correspond only to the coding region. Here, complete coding sequences of mouse, rat and dog Fgf11 orthologs were determined by using bioinformatics. Mouse Fgf11, rat Fgf11 and dog Fgf11 genes, consisting of five exons, were located within AL603707.5, AC098923.8 and AC126237.5 genome sequences, respectively. Human FGF11 showed 97.8%, 96.9%, 96.9% and 68.9% total-amino-acid identity with dog, mouse, rat and zebrafish Fgf11 orthologs, respectively. Rat Fgf11 showed Val205Met amino-acid substitution compared with rat Fgf11 RefSeq NP_570829.1. Met 205 of rat Fgf11 determined in this study was conserved among vertebrate Fgf11 orthologs. N-terminal signal peptide was not identified within vertebrate Fgf11 orthologs. Two Asn-linked glycosylation sites were identified within zebrafish fgf11, but not within mammalian Fgf11 orthologs. The 5'-flanking region, exonic regions, and intronic regions except intron 3 were well conserved between human FGF11 and mouse Fgf11 genes. Match program revealed that EVI1, ELK1, AP1 and E47-binding sites were conserved between human FGF11 and mouse Fgf11 promoter regions. Human FGF11 mRNA was expressed in embryonic stem (ES) cells, neuroblastoma, retinoblastoma, and brain tumors. This is the first report on the dog Fgf11 gene as well as on comparative genomics analyses of Fgf11 orthologs.
人类FGF19是啮齿动物Fgf15的直系同源基因，人类基因组和啮齿动物基因组中均存在22个FGF家族基因。小鼠Fgf11的参考序列NM_010198.1和大鼠Fgf11的参考序列NM_130816.1仅对应编码区。在此，通过生物信息学确定了小鼠、大鼠和犬Fgf11直系同源基因的完整编码序列。小鼠Fgf11、大鼠Fgf11和犬Fgf11基因均由五个外显子组成，分别位于AL603707.5、AC098923.8和AC126237.5基因组序列中。人类FGF11与犬、小鼠、大鼠和斑马鱼Fgf11直系同源基因的总氨基酸同一性分别为97.8%、96.9%、96.9%和68.9%。与大鼠Fgf11参考序列NP_570829.1相比，大鼠Fgf11存在Val205Met氨基酸替换。本研究确定的大鼠Fgf11的Met 205在脊椎动物Fgf11直系同源基因中保守。在脊椎动物Fgf11直系同源基因中未鉴定到N端信号肽。在斑马鱼fgf11中鉴定到两个N-糖基化位点，但在哺乳动物Fgf11直系同源基因中未鉴定到。人类FGF11和小鼠Fgf11基因之间，5'侧翼区、外显子区以及除内含子3外的内含子区均高度保守。比对程序显示，人类FGF11和小鼠Fgf11启动子区域之间EVI1、ELK1、AP1和E47结合位点保守。人类FGF11 mRNA在胚胎干细胞、神经母细胞瘤、视网膜母细胞瘤和脑肿瘤中表达。这是关于犬Fgf11基因以及Fgf11直系同源基因比较基因组学分析的首次报道。
